Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
acryloyl-CoA | apparent value, Km less than 0.01 mM, recombinant enzyme, at pH 7.5 and 22°C | Sulfurisphaera tokodaii | |
0.01 | - |
acryloyl-CoA | apparent value, Km less than 0.01 mM, recombinant enzyme, at pH 7.5 and 22°C | Metallosphaera sedula | |
0.036 | - |
NADPH | apparent value, recombinant enzyme, at pH 7.5 and 22°C | Metallosphaera sedula | |
0.036 | - |
acryloyl-CoA | apparent value, recombinant enzyme, at pH 7.5 and 22°C | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains 0.8 mol of Zn2+ per mol of enzyme monomer | Sulfurisphaera tokodaii | |
Zn2+ | contains 0.8 mol of Zn2+ per mol of enzyme monomer | Metallosphaera sedula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
calculated from amino acid sequence | Sulfurisphaera tokodaii |
40000 | - |
1 * 40000, SDS-PAGE | Sulfurisphaera tokodaii |
40000 | - |
1 * 40000, SDS-PAGE | Metallosphaera sedula |
43000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | - |
- |
- |
Sulfurisphaera tokodaii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat precipitation and Ni2+-chelating Sepharose column chromatography | Sulfurisphaera tokodaii |
heat precipitation, Q Sepharose column chromatography, carboxymethylcellulose column chromatography, ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Resource-S gel filtration | Metallosphaera sedula |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.014 | - |
cell extract, at pH 7.5 and 22°C | Metallosphaera sedula |
2.9 | - |
after 210fold purification, at pH 7.5 and 22°C | Metallosphaera sedula |
4.6 | - |
cell extract, at pH 7.5 and 22°C | Sulfurisphaera tokodaii |
18.7 | - |
after 4.1fold purification, at pH 7.5 and 22°C | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acryloyl-CoA + NADPH + H+ | - |
Metallosphaera sedula | propanoyl-CoA + NADP+ | - |
ir | |
acryloyl-CoA + NADPH + H+ | 100% specificity | Sulfurisphaera tokodaii | propanoyl-CoA + NADP+ | - |
ir | |
additional information | less than 1% specificity for crotonyl-CoA | Sulfurisphaera tokodaii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Sulfurisphaera tokodaii |
monomer | 1 * 40000, SDS-PAGE | Metallosphaera sedula |
Synonyms | Comment | Organism |
---|---|---|
acryloyl-CoA reductase | - |
Sulfurisphaera tokodaii |
acryloyl-CoA reductase | - |
Metallosphaera sedula |
acryloyl-CoA reductase (NADPH) | - |
Sulfurisphaera tokodaii |
acryloyl-CoA reductase (NADPH) | - |
Metallosphaera sedula |
acryloyl-coenzyme A reductase | - |
Sulfurisphaera tokodaii |
acryloyl-coenzyme A reductase | - |
Metallosphaera sedula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
acryloyl-CoA | apparent value, recombinant enzyme, at pH 7.5 and 22°C | Sulfurisphaera tokodaii | |
13 | - |
acryloyl-CoA | apparent value, recombinant enzyme, at pH 7.5 and 22°C | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Sulfurisphaera tokodaii | |
FAD | - |
Metallosphaera sedula | |
NADPH | - |
Sulfurisphaera tokodaii | |
NADPH | - |
Metallosphaera sedula |