Literature summary for 1.3.1.9 extracted from

Chollet, A.; Mourey, L.; Lherbet, C.; Delbot, A.; Julien, S.; Baltas, M.; Bernadou, J.; Pratviel, G.; Maveyraud, L.; Bernardes-Genisson, V.
Crystal structure of the enoyl-ACP reductase of Mycobacterium tuberculosis (InhA) in the apo-form and in complex with the active metabolite of isoniazid pre-formed by a biomimetic approach (2015), J. Struct. Biol., 190, 328-337.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoenzyme by the sitting drop vapor diffusion method using 100 mM sodium acetate, 100 mM MES pH 6.5 and 30% (w/v) polyethyleneglycol 400 or enzyme in complex with isoniazid, NAD+ or NADH by the hanging drop vapor diffusion method using 100 mM HEPES pH 7.5, 8-10% (w/v) 2-methyl-2,4-pentanediol, 50 mM sodium citrate pH 6.5	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
isoniazid	-	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+	Mycobacterium tuberculosis	-	an acyl-[acyl-carrier protein] + NAD+	-	?
a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+	Mycobacterium tuberculosis H37Rv	-	an acyl-[acyl-carrier protein] + NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WGR1	-	-
Mycobacterium tuberculosis H37Rv	P9WGR1	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-chelated His-Trap column chromatography, and Superdex 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+	-	Mycobacterium tuberculosis	an acyl-[acyl-carrier protein] + NAD+	-	?
a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+	-	Mycobacterium tuberculosis H37Rv	an acyl-[acyl-carrier protein] + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
enoyl-ACP reductase	-	Mycobacterium tuberculosis
InhA	-	Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
57.6	-	melting temperature	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)