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Literature summary for 1.3.1.9 extracted from

  • Heath, R.J.; Su, N.; Murphy, C.K.; Rock, C.O.
    The enoyl-[acyl-carrier-protein] reductases FabI and FabL from Bacillus subtilis (2000), J. Biol. Chem., 275, 40128-40133.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Bacillus subtilis
expression in Escherichia coli Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information the fabI knockout is as sensitive as the wild-type strain to the inhibitor triclosan Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
triclosan a slow-binding inhibitor of bsFabI and formed a stable bsFabI-NAD+-triclosan ternary complex Bacillus subtilis
[5-chloro-2-(2,4-dichlorophenoxy)phenol] 50% inhibition at 0.016 mM, builds a stable complex with FabI and NAD+, but inhibition of YgaA is reversible Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
NADH
-
Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis key enzyme in type II fatty-acid synthases that catalyzes the last step in each elongation cycle ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis P54616
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ chelation chromatography Bacillus subtilis
two enzymes: FabI and YgaA Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
FabI protein Bacillus subtilis
0.14
-
spectrophotometric assay, S-((2E)-oct-2-enoyl)-N-acetylcysteamine as a substrate Bacillus subtilis
0.18
-
YgaA protein Bacillus subtilis
2.3
-
spectrophotometric assay, (2E)-but-2-enoyl-[acyl carrier protein] as a substrate Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-but-2-enoyl-[acyl carrier protein] + NADH + H+
-
Bacillus subtilis butanoyl-[acyl-carrier protein] + NAD+
-
?
crotonoyl-CoA + NADH + H+
-
Bacillus subtilis butyryl-CoA + NAD+
-
?
additional information key enzyme in type II fatty-acid synthases that catalyzes the last step in each elongation cycle Bacillus subtilis ?
-
?
S-((2E)-oct-2-enoyl)-N-acetylcysteamine + NADH + H+
-
Bacillus subtilis S-octanoyl-N-acetylcysteamine + NAD+
-
?

Synonyms

Synonyms Comment Organism
bsFabI
-
Bacillus subtilis
enoyl-ACP reductase
-
Bacillus subtilis
enoyl-[acyl-carrier-protein] reductase
-
Bacillus subtilis
FabI
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NADH FabI protein Bacillus subtilis
NADH inactive with NADPH Bacillus subtilis
NADPH YgaA protein Bacillus subtilis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.016
-
-
Bacillus subtilis triclosan

General Information

General Information Comment Organism
malfunction physiological function of the enzyme is examined by knocking out the gene and determining the effect of the gene disruptions on cell growth and triclosan sensitivity, the gene is not essential Bacillus subtilis