Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
expression in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the fabI knockout is as sensitive as the wild-type strain to the inhibitor triclosan | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
triclosan | a slow-binding inhibitor of bsFabI and formed a stable bsFabI-NAD+-triclosan ternary complex | Bacillus subtilis | |
[5-chloro-2-(2,4-dichlorophenoxy)phenol] | 50% inhibition at 0.016 mM, builds a stable complex with FabI and NAD+, but inhibition of YgaA is reversible | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NADH | - |
Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus subtilis | key enzyme in type II fatty-acid synthases that catalyzes the last step in each elongation cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis | P54616 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ chelation chromatography | Bacillus subtilis |
two enzymes: FabI and YgaA | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
FabI protein | Bacillus subtilis |
0.14 | - |
spectrophotometric assay, S-((2E)-oct-2-enoyl)-N-acetylcysteamine as a substrate | Bacillus subtilis |
0.18 | - |
YgaA protein | Bacillus subtilis |
2.3 | - |
spectrophotometric assay, (2E)-but-2-enoyl-[acyl carrier protein] as a substrate | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-but-2-enoyl-[acyl carrier protein] + NADH + H+ | - |
Bacillus subtilis | butanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
crotonoyl-CoA + NADH + H+ | - |
Bacillus subtilis | butyryl-CoA + NAD+ | - |
? | |
additional information | key enzyme in type II fatty-acid synthases that catalyzes the last step in each elongation cycle | Bacillus subtilis | ? | - |
? | |
S-((2E)-oct-2-enoyl)-N-acetylcysteamine + NADH + H+ | - |
Bacillus subtilis | S-octanoyl-N-acetylcysteamine + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bsFabI | - |
Bacillus subtilis |
enoyl-ACP reductase | - |
Bacillus subtilis |
enoyl-[acyl-carrier-protein] reductase | - |
Bacillus subtilis |
FabI | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | FabI protein | Bacillus subtilis | |
NADH | inactive with NADPH | Bacillus subtilis | |
NADPH | YgaA protein | Bacillus subtilis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.016 | - |
- |
Bacillus subtilis | triclosan |
General Information | Comment | Organism |
---|---|---|
malfunction | physiological function of the enzyme is examined by knocking out the gene and determining the effect of the gene disruptions on cell growth and triclosan sensitivity, the gene is not essential | Bacillus subtilis |