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Literature summary for 1.3.1.9 extracted from
- Purification and characterizations of beta-ketoacyl-[acyl-carrier-protein] reductase, beta-hydroxyacyl-[acylcarrier-protein] dehydrase, and enoyl-[acyl-carrier-protein] reductase from Spinacia oleracea leaves (1982), Arch. Biochem. Biophys., 218, 77-91.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: arsenite | Spinacia oleracea | |
| N-ethylmaleimide | not | Spinacia oleracea |  |
| p-chloromercuribenzoate | 87% inhibition at 0.1 mM | Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0029 | - |
2-hexadecenoyl-[acyl-carrier protein] | - |
Spinacia oleracea | |
| 0.00552 | - |
2-decenoyl-[acyl-carrier protein] | - |
Spinacia oleracea | |
| 0.0062 | - |
2-hexenoyl-[acyl-carrier protein] | - |
Spinacia oleracea | |
| 0.0097 | - |
crotonyl-[acyl-carrier protein] | - |
Spinacia oleracea | |
| 0.011 | - |
NADH | - |
Spinacia oleracea | |
| 0.0133 | - |
2-octenoyl-[acyl-carrier protein] | - |
Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32500 | - |
monomeric form, SDS-PAGE | Spinacia oleracea |
| 115000 | - |
gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 63.69 | - |
- |
Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 2 months | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-decenoyl-[acyl-carrier protein] + NADH | - |
Spinacia oleracea | decanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
| 2-dodecenoyl-[acyl-carrier protein] + NADH | - |
Spinacia oleracea | dodecanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
| 2-hexadecenoyl-[acyl-carrier protein] + NADH | - |
Spinacia oleracea | hexadecanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
| 2-hexenoyl-[acyl-carrier protein] + NADH | fast reduction | Spinacia oleracea | hexanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
| 2-octenoyl-[acyl-carrier protein] + NADH | fast reduction | Spinacia oleracea | octanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
| crotonyl-[acyl-carrier protein] + NADH | - |
Spinacia oleracea | butyryl-[acyl-carrier protein] + NAD+ | - |
? | |
| additional information | no reduction of 3-decenoyl-[acyl-carrier-protein] | Spinacia oleracea | ? | - |
? | |
| additional information | substrate specificity: acyl chain length C4-C16 | Spinacia oleracea | ? | - |
? | |
| trans-2-decenoyl-[acyl-carrier protein] + NADH | - |
Spinacia oleracea | decanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 32500, SDS-PAGE | Spinacia oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Spinacia oleracea | |
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