Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28500 | - |
calculated from amino acid sequence | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephadex G-25M gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phenylpropionate + NAD+ | - |
Escherichia coli | 3-(2,3-dihydroxyphenyl) propionate + NADH + H+ | - |
? | |
cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD+ | - |
Escherichia coli | ? + NADH + H+ | - |
ir | |
cis-3-(2-carboxyethyl)-3,5-cyclohexadien-1,2-diol + NAD+ | - |
Escherichia coli | 3-(2,3-dihydroxyphenyl)propionate + NADH + H+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase | - |
Escherichia coli |
3-phenylpropionate-2',3'-dihydrodiol dehydrogenase | - |
Escherichia coli |
3-phenylpropionate-dihydrodiol dehydrogenase | - |
Escherichia coli |
HcaB | - |
Escherichia coli |
HCAR | HcaR controls expression of the neighbouring genes, hcaA1, hcaA2, hcaC, hcaB and hcaD, coding for the 3-phenylpropionate dioxygenase complex and 3-phenylpropionate-2',3'-dihydrodiol dehydrogenase | Escherichia coli |
Organism | Comment | Expression |
---|---|---|
Escherichia coli | when bacteria are grown in the absence of oxygen, the expression of hcaR is decreased threefold compared to growth in aerobic conditions | down |