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Literature summary for 1.3.1.85 extracted from

  • Zhang, L.; Chen, J.; Mori, T.; Yan, Y.; Liu, W.; Abe, I.
    Crystallization and preliminary X-ray diffraction analysis of AntE, a crotonyl-CoA carboxylase/reductase from Streptomyces sp. NRRL 2288 (2014), Acta Crystallogr. Sect. F, 70, 734-737.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BLR(DE3) cells Streptomyces sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM CAPS-NaOH pH 10.0, 38% (v/v) PEG 400 Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
4 * 47000, SDS-PAGE Streptomyces sp.
143000
-
gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
crotonyl-CoA + CO2 + NADPH + H+ Streptomyces sp.
-
(2S)-ethylmalonyl-CoA + NADP+
-
?
crotonyl-CoA + CO2 + NADPH + H+ Streptomyces sp. NRRL 2288
-
(2S)-ethylmalonyl-CoA + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. M1SQA1
-
-
Streptomyces sp. NRRL 2288 M1SQA1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200HR gel filtration Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-3-cyclohexylprop-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. (2S)-2-carboxy-3-cyclohexylpropanoyl-CoA + NADP+
-
?
(2E)-3-cyclohexylprop-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. NRRL 2288 (2S)-2-carboxy-3-cyclohexylpropanoyl-CoA + NADP+
-
?
(2E)-4-methylhex-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. [(2R)-2-methylbutyl]propanedioyl-CoA + NADP+
-
?
(2E)-4-methylhex-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. NRRL 2288 [(2R)-2-methylbutyl]propanedioyl-CoA + NADP+
-
?
(2E)-5-chloropent-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. (2S)-2-carboxy-5-chloropentanoyl-CoA + NADP+
-
?
(2E)-5-chloropent-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. NRRL 2288 (2S)-2-carboxy-5-chloropentanoyl-CoA + NADP+
-
?
(2E)-5-methylhex-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. (2S)-(3-methylbutyl)propanedioyl-CoA + NADP+
-
?
(2E)-5-methylhex-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. NRRL 2288 (2S)-(3-methylbutyl)propanedioyl-CoA + NADP+
-
?
(2E)-6-methyloct-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. (2S)-2-carboxy-6-methyloctanoyl-CoA + NADP+
-
?
(2E)-hept-2-en-6-ynoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. (2S)-2-craboxy-hept-6-ynoyl-CoA + NADP+
-
?
(2E)-hex-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. (S)-2-butylpropanedioyl-CoA + NADP+
-
?
(2E)-oct-2-enoyl-CoA + NADPH + CO2 + H+
-
Streptomyces sp. (2S)-2-carboxyoctanoyl-CoA + NADP+
-
?
crotonyl-CoA + CO2 + NADPH + H+
-
Streptomyces sp. (2S)-ethylmalonyl-CoA + NADP+
-
?
crotonyl-CoA + CO2 + NADPH + H+
-
Streptomyces sp. NRRL 2288 (2S)-ethylmalonyl-CoA + NADP+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 47000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
AntE
-
Streptomyces sp.
CCR
-
Streptomyces sp.
crotonyl-CoA carboxylase/reductase
-
Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Streptomyces sp.