Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cereibacter sphaeroides 2.4.1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
CO2 | in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication | Cereibacter sphaeroides 2.4.1 | |
0.2 | - |
(2S)-ethylmalonyl-CoA | in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication | Cereibacter sphaeroides 2.4.1 | |
0.4 | - |
crotonyl-CoA | in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication | Cereibacter sphaeroides 2.4.1 | |
0.7 | - |
NADPH | in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication | Cereibacter sphaeroides 2.4.1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals | Methylorubrum extorquens AM1 | |
additional information | comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals | Cereibacter sphaeroides 2.4.1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47700 | - |
2 * 47700, gel filtration | Cereibacter sphaeroides 2.4.1 |
105000 | - |
gel filtration | Cereibacter sphaeroides 2.4.1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides 2.4.1 | - |
- |
- |
Methylorubrum extorquens AM1 | - |
- |
- |
Streptomyces coelicolor | - |
strain A3 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and Cibacron Blue 3GA agarose column chromatography | Cereibacter sphaeroides 2.4.1 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate-grown cell | - |
Cereibacter sphaeroides 2.4.1 | - |
culture condition:butyrate-grown cell | - |
Streptomyces coelicolor | - |
culture condition:methanol-grown cell | - |
Methylorubrum extorquens AM1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + CO2 + NADPH + H+ | crotonyl-CoA is the best substrate | Streptomyces coelicolor | (2S)-ethylmalonyl-CoA + NADP+ | - |
r | |
crotonyl-CoA + CO2 + NADPH + H+ | crotonyl-CoA is the best substrate | Methylorubrum extorquens AM1 | (2S)-ethylmalonyl-CoA + NADP+ | - |
r | |
crotonyl-CoA + CO2 + NADPH + H+ | crotonyl-CoA is the best substrate | Cereibacter sphaeroides 2.4.1 | (2S)-ethylmalonyl-CoA + NADP+ | - |
r | |
crotonyl-CoA + NADPH + H+ | reduction at low rate | Streptomyces coelicolor | butyryl-CoA + NADP+ | - |
ir | |
crotonyl-CoA + NADPH + H+ | reduction at low rate | Methylorubrum extorquens AM1 | butyryl-CoA + NADP+ | - |
ir | |
crotonyl-CoA + NADPH + H+ | reduction at low rate | Cereibacter sphaeroides 2.4.1 | butyryl-CoA + NADP+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47700, gel filtration | Cereibacter sphaeroides 2.4.1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
104 | - |
(2S)-ethylmalonyl-CoA | in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication | Cereibacter sphaeroides 2.4.1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Streptomyces coelicolor | |
NADPH | - |
Methylorubrum extorquens AM1 | |
NADPH | - |
Cereibacter sphaeroides 2.4.1 |
General Information | Comment | Organism |
---|---|---|
metabolism | crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway | Streptomyces coelicolor |
metabolism | crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway | Methylorubrum extorquens AM1 |
metabolism | crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway | Cereibacter sphaeroides 2.4.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
520 | - |
(2S)-ethylmalonyl-CoA | in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication | Cereibacter sphaeroides 2.4.1 |