Cloned (Comment) | Organism |
---|---|
gene LYL1, map-based cloning of LYL1, phylogenetic analysis, quantitative real-time PCR enzyme expression analysis | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
additional information | analysis of properties and phenotype of mutant lyl1-1, the mutation of LYL1 leads to an accumulation of Chl intermediates and a deficiency of alpha-tocopherol, overview. The C-to-T substitution results in a change from an alanine residue to valine in the encoded protein in lyl1-1. Enzyme knockout by RNA interference, phenotype of a 3-week-old RNAi transgenic plant | Oryza sativa Japonica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + 3 NADPH + 3 H+ | Oryza sativa Japonica Group | - |
phytyl diphosphate + 3 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | U5LY03 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + 3 NADPH + 3 H+ | - |
Oryza sativa Japonica Group | phytyl diphosphate + 3 NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, about, sequence calculation | Oryza sativa Japonica Group |
Synonyms | Comment | Organism |
---|---|---|
geranylgeranyl reductase | - |
Oryza sativa Japonica Group |
LYL1 | - |
Oryza sativa Japonica Group |
NADPH-dependent geranylgeranyl reductase | - |
Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme has a FAD binding domain | Oryza sativa Japonica Group | |
NADPH | dependent on | Oryza sativa Japonica Group |
Organism | Comment | Expression |
---|---|---|
Oryza sativa Japonica Group | LYL1 is induced by light and suppressed by darkness which is consistent with its potential biological functions | down |
Oryza sativa Japonica Group | LYL1 is induced by light and suppressed by darkness which is consistent with its potential biological functions | up |
General Information | Comment | Organism |
---|---|---|
malfunction | the light-induced yellow leaf 1-1 (lyl1-1) mutant is hypersensitive to high-light and defective in the Chl synthesis. The mutation of LYL1 leads to conjugation of the majority of Chl molecules with an unsaturated geranylgeraniol side chain. The lyl1-1 mutant suffers from severe photooxidative damage and displays a drastic reduction in the levels of alpha-tocopherol and photosynthetic proteins. The yellowing of lyl1-1 mutant is caused by high-light stress. Mutant lyl1-1 phenotype, overview | Oryza sativa Japonica Group |
metabolism | the three-step hydrogenation of geranylgeranyl diphosphate into phytyl diphosphate, and Chl-geranylgeranylated (ChlGG) and Chl-phytol (ChlPhy) is catalyzed by NADPH-dependent geranylgeranyl reductase. ENzyme LYL1 protects against lipid peroxidation and reactive oxygen species, ROS | Oryza sativa Japonica Group |
physiological function | the enzyme is involved in the reduction step from Chl-geranylgeranylated (ChlGG) and geranylgeranyl diphosphate (GGPP) to Chl-phytol (ChlPhy) and phytyl diphosphate (PPP) in rice. Enzyme LYL1 also plays an important role in response to high-light in rice | Oryza sativa Japonica Group |