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Literature summary for 1.3.1.81 extracted from

  • Turner, G.W.; Croteau, R.
    Organization of monoterpene biosynthesis in Mentha. Immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase (2004), Plant Physiol., 136, 4215-4227.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Mentha x piperita

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Mentha x piperita
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000 Mentha x piperita

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 (+)-pulegone + 2 NADPH + 2 H+ Mentha x piperita the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview (-)-menthone + (+)-isomenthone + 2 NADP+
-
?

Organism

Organism UniProt Comment Textmining
Mentha x piperita
-
cv Black Mitcham
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision Mentha x piperita

Source Tissue

Source Tissue Comment Organism Textmining
secretory trichome secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development Mentha x piperita
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (+)-pulegone + 2 NADPH + 2 H+ the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview Mentha x piperita (-)-menthone + (+)-isomenthone + 2 NADP+
-
?
2 (+)-pulegone + 2 NADPH + 2 H+ the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio Mentha x piperita (-)-menthone + (+)-isomenthone + 2 NADP+
-
?

Subunits

Subunits Comment Organism
? x * 38000 Mentha x piperita

Synonyms

Synonyms Comment Organism
More the enzyme is a member of the medium-chain dehydrogenase/reductase superfamily Mentha x piperita
pulegone reductase
-
Mentha x piperita

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Mentha x piperita