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Literature summary for 1.3.1.8 extracted from

  • Cvetanovic, M.; Moreno de la Garza, M.; Dommes, V.; Kunau, W.H.
    Purification and characterization of 2-enoyl-CoA-reductase from bovine liver (1985), Biochem. J., 227, 49-56.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.8

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide
-
 Bos taurus
NADH in presence of NADPH 0.1 mM Bos taurus
NADP+
-
 Bos taurus
NADPH concentration above 0.1 mM Bos taurus
p-chloromercuribenzoate
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE, with assumed MW of about 70000 Bos taurus
59000
-
 gel filtration Bos taurus
68000
-
 sucrose density gradient centrifugation Bos taurus
98000
-
 gradient, native PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0117
-
 purified enzyme, substrate 2-trans,4-trans-hexadienoyl-CoA Bos taurus
0.0219
-
 purified enzyme, substrate 2-trans,4-trans-decadienoyl-CoA Bos taurus
0.29
-
 purified enzyme, substrate 2-trans-tetradecenoyl-CoA Bos taurus
1.42
-
 purified enzyme, substrate 2-trans-decenoyl-CoA Bos taurus
1.46
-
 purified enzyme, substrate 2-trans-octenoyl-CoA Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,4-trans-decadienoyl-CoA + NADPH low activity Bos taurus ?
-
 ?
2-trans,4-trans-hexadienoyl-CoA + NADPH low activity Bos taurus ?
-
 ?
2-trans-decenoyl-CoA + NAD(P)H
-
 Bos taurus decanoyl-CoA + NADP+
-
 ?
2-trans-dodecenoyl-CoA + NAD(P)H
-
 Bos taurus dodecanoyl-CoA + NAD(P)+
-
 ?
2-trans-hexenoyl-CoA + NAD(P)H
-
 Bos taurus hexanoyl-CoA + NADP+
-
 ?
2-trans-octenoyl-CoA + NAD(P)H
-
 Bos taurus octanoyl-CoA + NADP+
-
 ?
2-trans-tetradecenoyl-CoA + NADPH
-
 Bos taurus tetradecanoyl-CoA + NADP+
-
 ?
additional information no cis-isomeric substrates tested, classification is not definite Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE, with assumed MW of about 70000 Bos taurus

Synonyms

Synonyms Comment Organism
2-enoyl-CoA reductase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH inactive with NADH Bos taurus