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Literature summary for 1.3.1.8 extracted from
- Separation and some characterizations of NADPH-enoyl CoA reductase(s) from Candida albicans (1974), Chem. Pharm. Bull., 22, 2685-2691.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
2-trans-octenoyl-CoA | reductase type I | Candida albicans |  |
| 0.0011 | - |
2-cis-octenoyl-CoA | reductase type I | Candida albicans | |
| 0.0025 | - |
5-hydroxyundec-cis-2-enoyl-CoA | reductase type I | Candida albicans | |
| 0.003 | - |
5-hydroxyundec-cis-2-enoyl-CoA | reductase type II | Candida albicans | |
| 0.01 | - |
2-trans-octenoyl-CoA | reductase type II | Candida albicans | |
| 0.053 | - |
2-cis-octenoyl-CoA | reductase type II | Candida albicans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
reductase type II, gel filtration | Candida albicans |
| 180000 | - |
reductase type I, gel filtration | Candida albicans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxyundec-cis-2-enoyl-CoA + NADPH | Candida albicans | - |
5-hydroxyundecanoyl-CoA + NADP+ | - |
? | |
| additional information | Candida albicans | role in beta-oxidation of fatty acids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
reductase type I and II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| reductase type I and II | Candida albicans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-cis-octenoyl-CoA + NADPH | - |
Candida albicans | octanoyl-CoA + NADP+ | - |
? | |
| 2-trans-octenoyl-CoA + NAD(P)H | - |
Candida albicans | octanoyl-CoA + NADP+ | - |
? | |
| 5-hydroxyundec-cis-2-enoyl-CoA + NADPH | - |
Candida albicans | 5-hydroxyundecanoyl-CoA + NADP+ | - |
? | |
| additional information | role in beta-oxidation of fatty acids | Candida albicans | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
reductase type I: 30 min, 50% loss of activity, reductase type II: no activity with 5 hydroxyundec-cis-2-enoyl-CoA, up to 80% activity with cis or trans-oct-2-enoyl-CoA | Candida albicans |
| 60 | - |
reductase type I: 3 min, 80% loss of activity | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | depending on substrate and type of reductase | Candida albicans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | inactive with NADH | Candida albicans | |
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