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Literature summary for 1.3.1.77 extracted from

  • Liu, C.; Wang, X.; Shulaev, V.; Dixon, R.A.
    A role for leucoanthocyanidin reductase in the extension of proanthocyanidins (2016), Nat. Plants, 2, 16182 .
    View publication on PubMed
Search for more literature for 1.3.1.77

Cloned(Commentary)

Cloned (Comment) Organism
gene BAN, overexpression of the enzyme in Medicago truncatula hairy roots. Recombinant enzyme ANR does not generate epicatechin from epicatechin-cysteine conjugate in hairy roots Medicago truncatula

Protein Variants

Protein Variants Comment Organism
additional information construction of Tnt1 insertion mutants of gene ANR, two mutants are isolated with Tnt1 insertions in the third and sixth exons, respectively, leading to loss of function of ANR, which gives large reductions in both soluble and insoluble proanthocyanidins in seeds compared to wild-type, low amounts of epicatechin and its 3'-O-glucoside in the anr-1 mutant. The seeds of anr mutants are darkred resulting from redirected metabolic flow from anthocyanidin to anthocyanin. The lar-1/anr-1 double mutant displays the same seed colour as the anr-1 mutant. The anr mutant seeds contain leucocyandin for catechin production, but no substrate for epicatechin production Medicago truncatula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyanidin + 2 NADPH + H+ Medicago truncatula
-
 (2R,3R)-epicatechin + 2 NADP+ ? ?
cyanidin + 2 NADPH + H+ Medicago truncatula ecotype R108
-
 (2R,3R)-epicatechin + 2 NADP+ ? ?
additional information Medicago truncatula recombinant enzyme ANR does not generate epicatechin from epicatechin-cysteine conjugate in hairy roots ?
-
 ?
additional information Medicago truncatula ecotype R108 recombinant enzyme ANR does not generate epicatechin from epicatechin-cysteine conjugate in hairy roots ?
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago truncatula Q84XT1
-
-
Medicago truncatula ecotype R108 Q84XT1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hairy root
-
 Medicago truncatula
-
seed coat
-
 Medicago truncatula
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanidin + 2 NADPH + H+
-
 Medicago truncatula (2R,3R)-epicatechin + 2 NADP+ ? ?
cyanidin + 2 NADPH + H+
-
 Medicago truncatula ecotype R108 (2R,3R)-epicatechin + 2 NADP+ ? ?
additional information recombinant enzyme ANR does not generate epicatechin from epicatechin-cysteine conjugate in hairy roots Medicago truncatula ?
-
 ?
additional information recombinant enzyme ANR does not generate epicatechin from epicatechin-cysteine conjugate in hairy roots Medicago truncatula ecotype R108 ?
-
 ?

Synonyms

Synonyms Comment Organism
ANR
-
 Medicago truncatula
BAN
-
 Medicago truncatula

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Medicago truncatula

Expression

Organism Comment Expression
Medicago truncatula overexpression of the myeloblastosis (MYB)14 or MYB5 transcription factors induces proanthocyanidin biosynthesis in hairy roots, ANR is highly induced in MYB5 overexpressing hairy roots up

General Information

General Information Comment Organism
malfunction loss of function ANR mutants show large reductions in both soluble and insoluble proanthocyanidins in seeds compared to wild-type, as well as low amounts of epicatechin and its 3'-O-glucoside in the anr-1 mutant. The seeds of anr mutants are darkred resulting from redirected metabolic flow from anthocyanidin to anthocyanin Medicago truncatula