Literature summary for 1.3.1.75 extracted from

Parham, R.; Rebeiz, C.A.
Chloroplast biogenesis 72: a [4-vinyl]chlorophyllide a reductase assay using divinyl chlorophyllide a as an exogenous substrate (1995), Anal. Biochem., 231, 164-169.

Search for more literature for 1.3.1.75

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Hordeum vulgare	9507	-
chloroplast	-	Zea mays	9507	-
chloroplast	-	Cucumis sativus	9507	-
etioplast	-	Hordeum vulgare	9513	-
etioplast	-	Zea mays	9513	-
etioplast	-	Cucumis sativus	9513	-
membrane	-	Hordeum vulgare	16020	-
membrane	-	Zea mays	16020	-
membrane	-	Cucumis sativus	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,4-divinyl chlorophyllide a + NADPH + H+	Hordeum vulgare	-	2-vinyl-4-ethyl chlorophyllide a + NADP+	i.e. monovinyl chlorophyllide a	r
2,4-divinyl chlorophyllide a + NADPH + H+	Zea mays	-	2-vinyl-4-ethyl chlorophyllide a + NADP+	i.e. monovinyl chlorophyllide a	r
2,4-divinyl chlorophyllide a + NADPH + H+	Cucumis sativus	-	2-vinyl-4-ethyl chlorophyllide a + NADP+	i.e. monovinyl chlorophyllide a	r

Organism

Organism	UniProt	Comment	Textmining
Cucumis sativus	-	light-inducible	-
Cucumis sativus	-	Beit Alpha MR	-
Hordeum vulgare	-	light-inducible	-
Zea mays	-	light-inducible	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cotyledon	etiolated	Hordeum vulgare	-
cotyledon	etiolated	Zea mays	-
cotyledon	etiolated	Cucumis sativus	-
leaf	etiolated	Hordeum vulgare	-
leaf	etiolated	Zea mays	-
leaf	etiolated	Cucumis sativus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	assay development	Cucumis sativus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,4-divinyl chlorophyllide a + NADPH + H+	-	Hordeum vulgare	2-vinyl-4-ethyl chlorophyllide a + NADP+	i.e. monovinyl chlorophyllide a	r
2,4-divinyl chlorophyllide a + NADPH + H+	-	Zea mays	2-vinyl-4-ethyl chlorophyllide a + NADP+	i.e. monovinyl chlorophyllide a	r
2,4-divinyl chlorophyllide a + NADPH + H+	-	Cucumis sativus	2-vinyl-4-ethyl chlorophyllide a + NADP+	i.e. monovinyl chlorophyllide a	r
2,4-divinyl chlorophyllide a + NADPH + H+	strictly dependent on divinyl chlorophyllide a and NADPH, no activity with divinyl protochlorophyllide a, NADH and GSH	Hordeum vulgare	2-vinyl-4-ethyl chlorophyllide a + NADP+	i.e. monovinyl chlorophyllide a	r
2,4-divinyl chlorophyllide a + NADPH + H+	strictly dependent on divinyl chlorophyllide a and NADPH, no activity with divinyl protochlorophyllide a, NADH and GSH	Cucumis sativus	2-vinyl-4-ethyl chlorophyllide a + NADP+	i.e. monovinyl chlorophyllide a	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Cucumis sativus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	50	about half-maximal activity at 10°C and 50°C	Cucumis sativus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	complete inactivation after 2 min	Cucumis sativus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.3	-	-	Hordeum vulgare
6.3	-	-	Zea mays
6.3	-	-	Cucumis sativus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	8.5	about half-maximal activity at pH 5.5 and pH 8.5, no activity below pH 5.2 and above pH 11.0	Cucumis sativus

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Release 2023.1 (January 2023)