Literature summary for 1.3.1.74 extracted from

Itoh, K.; Yamamoto, K.; Adachi, M.; Kosaka, T.; Tanaka, Y.
Leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin DELTA13-reductase (LTB4 12-HD/PGR) responsible for the reduction of a double-bond of the alpha,beta-unsaturated ketone of an aryl propionic acid non-steroidal anti-inflammatory agent CS-670 (2008), Xenobiotica, 38, 249-263.

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Activating Compound

Activating Compound	Comment	Organism	Structure
ammonium sulfate	stimulates activity	Rattus norvegicus
CH3COO- NH4+	stimulates activity	Rattus norvegicus
CH3COONa	stimulates activity	Rattus norvegicus
Na2S2O5	stimulates activity	Rattus norvegicus
Na2SO4	stimulates activity	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Top10 cells	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
15-keto prostaglandin F2alpha	43.06% residual activity at 0.1 mM	Rattus norvegicus
benzylidene acetophenone	21.09% residual activity at 0.5 mM	Rattus norvegicus
dicoumarol	11.45% residual activity at 0.1 mM	Rattus norvegicus
indomethacin	14.22% residual activity at 0.1 mM	Rattus norvegicus
additional information	finasteride has no inhibitory effect	Rattus norvegicus
quercitrin	13.14% residual activity at 0.1 mM	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid	at 30°C	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	stimulates activity	Rattus norvegicus
K+	stimulates activity	Rattus norvegicus
Li+	stimulates activity	Rattus norvegicus
Mg2+	stimulates activity	Rattus norvegicus
Mn2+	stimulates activity	Rattus norvegicus
Na+	stimulates activity	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	SDS-PAGE	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
2',5'-ADP Sepharose 4B column chromatography, Resource Q column chromatography, and HiLoad Superdex gel filtration	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0054	-	cytosol extract	Rattus norvegicus
0.803	-	after 150fold purification	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid + NADPH	2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid is also known as CS-670	Rattus norvegicus	2-[4-[(2-oxocyclohexyl)methyl]phenyl]propanoic acid + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase	a beta-NADPH-dependent alkenal/one oxidoreductase which has a role for leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandinDELTA13-reductase	Rattus norvegicus
CS-670 double-bond reductase	-	Rattus norvegicus
LTB4 12-HD/PGR	-	Rattus norvegicus
NADPH-dependent alkenal/one oxidoreductase	-	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	dependent	Rattus norvegicus

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Release 2023.1 (January 2023)