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Literature summary for 1.3.1.74 extracted from

  • Gong, J.; Neels, J.F.; Yu, X.; Kensler, T.W.; Peterson, L.A.; Sturla, S.J.
    Investigating the role of stereochemistry in the activity of anticancer acylfulvenes: synthesis, reductase-mediated bioactivation, and cellular toxicity (2006), J. Med. Chem., 49, 2593-2599.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
human embryonic kidney cells (293 cell line) are transiently transfected either with an episomal rAOR overexpression vector (pCEP4-rAOR) or control vector (pCEP4). AOR transfected cells produce a 7-8fold higher expression of AOR than control cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.213
-
(-)-hydroxymethylacylfulvene pH 7.2, 37°C Homo sapiens
0.243
-
(+)-hydroxymethylacylfulvene pH 7.2, 37°C Homo sapiens
0.465
-
(+)-acylfulvene pH 7.2, 37°C Homo sapiens
0.538
-
(-)-acylfulvene pH 7.2, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-acylfulvene + NADPH
-
Homo sapiens ?
-
?
(+)-hydroxymethylacylfulvene + NADPH
-
Homo sapiens ?
-
?
(-)-acylfulvene + NADPH
-
Homo sapiens ?
-
?
(-)-hydroxymethylacylfulvene + NADPH
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
alkenal/one reductase
-
Homo sapiens
AOR
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens