BRENDA - Enzyme Database show
show all sequences of 1.3.1.71

Biosynthesis of steroids and terpenoids. XI. 24-Methylene sterol 24(28)-reductase of Saccharomyces cerevisiae

Jarman, T.R.; Gunatilaka, A.A.L.; Widdowson, D.A.; Bioorg. Chem. 4, 202-211 (1975)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
25% glycerol and cofactor NADPH or NADH at 1.3 mM stabilize the enzyme
Saccharomyces cerevisiae
anaerobiosis leads to loss of 15% activity after 24 h
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Saccharomyces cerevisiae
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
Saccharomyces cerevisiae
terminal step in ergosterol biosynthesis, pathway overview, presursor analysis
ergosterol + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
partially
Saccharomyces cerevisiae
Storage Stability
Storage Stability
Organism
0°C, after 24 h: completely stable in presence of NADPH or NADH, 42% remaining activity in presence of 10 mM GSH or 2-mercaptoethanol, 33% remaining activity without additives, after 48 h: 93% remianing activity in prsence of 25% glycerol, 37% with 10% glycerol, and 13% remaining activity without additives
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
preferred substrate
654951
Saccharomyces cerevisiae
ergosterol + NADP+
-
-
-
?
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
terminal step in ergosterol biosynthesis, pathway overview, presursor analysis
654951
Saccharomyces cerevisiae
ergosterol + NADP+
-
-
-
?
additional information
ergosta-7,22,24(28)-trien-3beta-ol, episterol, and fecosterol are poor substrates, 24,25-dihydro-24-methylene lanosterol is no substrate
654951
Saccharomyces cerevisiae
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
about 10fold less active than NADPH
Saccharomyces cerevisiae
NADPH
preferred cofactor
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
about 10fold less active than NADPH
Saccharomyces cerevisiae
NADPH
preferred cofactor
Saccharomyces cerevisiae
General Stability (protein specific)
General Stability
Organism
25% glycerol and cofactor NADPH or NADH at 1.3 mM stabilize the enzyme
Saccharomyces cerevisiae
anaerobiosis leads to loss of 15% activity after 24 h
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Saccharomyces cerevisiae
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
Saccharomyces cerevisiae
terminal step in ergosterol biosynthesis, pathway overview, presursor analysis
ergosterol + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability
Organism
0°C, after 24 h: completely stable in presence of NADPH or NADH, 42% remaining activity in presence of 10 mM GSH or 2-mercaptoethanol, 33% remaining activity without additives, after 48 h: 93% remianing activity in prsence of 25% glycerol, 37% with 10% glycerol, and 13% remaining activity without additives
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
preferred substrate
654951
Saccharomyces cerevisiae
ergosterol + NADP+
-
-
-
?
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
terminal step in ergosterol biosynthesis, pathway overview, presursor analysis
654951
Saccharomyces cerevisiae
ergosterol + NADP+
-
-
-
?
additional information
ergosta-7,22,24(28)-trien-3beta-ol, episterol, and fecosterol are poor substrates, 24,25-dihydro-24-methylene lanosterol is no substrate
654951
Saccharomyces cerevisiae
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 1.3.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745462
Kaneshiro
Sterols of Saccharomyces cere ...
Pneumocystis carinii, Pneumocystis carinii B80
J. Eukaryot. Microbiol.
62
298-306
2015
-
-
1
-
1
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684606
He
Ergosterol production from mol ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae YEH56(pHXA42)
Appl. Microbiol. Biotechnol.
75
55-60
2007
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654205
Visbal
S-adenosyl-L-methionine inhibi ...
Paracoccidioides brasiliensis
Antimicrob. Agents Chemother.
47
2966-2970
2003
-
-
-
-
-
-
4
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390848
Zweytick
Biochemical characterization a ...
Saccharomyces cerevisiae
FEBS Lett.
470
83-87
2000
-
-
1
-
-
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657077
Salimova
-
Sterol mutants of Chlamydomona ...
Chlamydomonas reinhardtii, Chlamydomonas reinhardtii arg7cw15
Plant Physiol. Biochem.
37
241-249
1999
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390847
Pierce
Azasterol inhibitors in yeast. ...
Saccharomyces cerevisiae
Can. J. Biochem.
57
201-208
1979
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390846
Pierce
Azasterol inhibitors in yeast. ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
529
429-437
1978
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390845
Neal
Sterol 24(28) methylene reduct ...
Saccharomyces cerevisiae
J. Bacteriol.
129
1375-1378
1977
1
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
654951
Jarman
-
Biosynthesis of steroids and t ...
Saccharomyces cerevisiae
Bioorg. Chem.
4
202-211
1975
-
-
-
-
-
2
-
-
1
-
-
1
-
1
-
-
1
-
-
-
-
1
3
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
1
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-