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show all sequences of 1.3.1.71

Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, Erg4p, from the yeast Saccharomyces cerevisiae

Zweytick, D.; Hrastnik, C.; Kohlwein, S.D.; Daum, G.; FEBS Lett. 470, 83-87 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
green fluorescence protein fusion protein expressed in Saccharomyces cerevisiae erg4 mutant
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Saccharomyces cerevisiae
5783
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
x * 56000, deduced from immunoblot
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
Saccharomyces cerevisiae
-
ergosta-5,7,22-trien-3beta-ol + NADP+
-
Saccharomyces cerevisiae
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00032
-
enzyme activity in 40000 x g microsomal fraction, corresponding to endoplasmic reticulum membranes
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
390848
Saccharomyces cerevisiae
ergosta-5,7,22-trien-3beta-ol + NADP+
-
390848
Saccharomyces cerevisiae
?
ergosta-5,7,22-trien-3beta-ol + NADP+
-
390848
Saccharomyces cerevisiae
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
390848
Saccharomyces cerevisiae
?
Subunits
Subunits
Commentary
Organism
?
x * 56000, deduced from immunoblot
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
green fluorescence protein fusion protein expressed in Saccharomyces cerevisiae erg4 mutant
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Saccharomyces cerevisiae
5783
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
x * 56000, deduced from immunoblot
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
Saccharomyces cerevisiae
-
ergosta-5,7,22-trien-3beta-ol + NADP+
-
Saccharomyces cerevisiae
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00032
-
enzyme activity in 40000 x g microsomal fraction, corresponding to endoplasmic reticulum membranes
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
390848
Saccharomyces cerevisiae
ergosta-5,7,22-trien-3beta-ol + NADP+
-
390848
Saccharomyces cerevisiae
?
ergosta-5,7,22-trien-3beta-ol + NADP+
-
390848
Saccharomyces cerevisiae
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
390848
Saccharomyces cerevisiae
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 56000, deduced from immunoblot
Saccharomyces cerevisiae
Other publictions for EC 1.3.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745462
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390848
Zweytick
Biochemical characterization a ...
Saccharomyces cerevisiae
FEBS Lett.
470
83-87
2000
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1
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657077
Salimova
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Sterol mutants of Chlamydomona ...
Chlamydomonas reinhardtii, Chlamydomonas reinhardtii arg7cw15
Plant Physiol. Biochem.
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241-249
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390847
Pierce
Azasterol inhibitors in yeast. ...
Saccharomyces cerevisiae
Can. J. Biochem.
57
201-208
1979
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390846
Pierce
Azasterol inhibitors in yeast. ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
529
429-437
1978
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390845
Neal
Sterol 24(28) methylene reduct ...
Saccharomyces cerevisiae
J. Bacteriol.
129
1375-1378
1977
1
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654951
Jarman
-
Biosynthesis of steroids and t ...
Saccharomyces cerevisiae
Bioorg. Chem.
4
202-211
1975
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