Literature summary for 1.3.1.71 extracted from

Zweytick, D.; Hrastnik, C.; Kohlwein, S.D.; Daum, G.
Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, Erg4p, from the yeast Saccharomyces cerevisiae (2000), FEBS Lett., 470, 83-87.

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Cloned(Commentary)

Cloned (Comment)	Organism
green fluorescence protein fusion protein expressed in Saccharomyces cerevisiae erg4 mutant	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	-	Saccharomyces cerevisiae	5783	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	x * 56000, deduced from immunoblot	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+	Saccharomyces cerevisiae	-	ergosta-5,7,22-trien-3beta-ol + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00032	-	enzyme activity in 40000 x g microsomal fraction, corresponding to endoplasmic reticulum membranes	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+	-	Saccharomyces cerevisiae	ergosta-5,7,22-trien-3beta-ol + NADP+	-	?
ergosta-5,7,22-trien-3beta-ol + NADP+	-	Saccharomyces cerevisiae	ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 56000, deduced from immunoblot	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)