BRENDA - Enzyme Database show
show all sequences of 1.3.1.71

Azasterol inhibitors in yeast. Inhibition of the DELTA24-sterol methyltransferase and the 24-methylene sterol DELTA24(28)-reductase in sterol mutants of Saccharomyces cerevisiae

Pierce, A.M.; Unrau, A.M.; Oehlschlager, A.C.; Woods, R.A.; Can. J. Biochem. 57, 201-208 (1979)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
23-Azacholesterol
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
Saccharomyces cerevisiae
-
ergosta-5,7,22-trien-3beta-ol + NADP+
-
Saccharomyces cerevisiae
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
390847
Saccharomyces cerevisiae
ergosta-5,7,22-trien-3beta-ol + NADP+
-
390847
Saccharomyces cerevisiae
?
ergosta-5,7,22-trien-3beta-ol + NADP+
-
390847
Saccharomyces cerevisiae
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
390847
Saccharomyces cerevisiae
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
23-Azacholesterol
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
Saccharomyces cerevisiae
-
ergosta-5,7,22-trien-3beta-ol + NADP+
-
Saccharomyces cerevisiae
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
390847
Saccharomyces cerevisiae
ergosta-5,7,22-trien-3beta-ol + NADP+
-
390847
Saccharomyces cerevisiae
?
ergosta-5,7,22-trien-3beta-ol + NADP+
-
390847
Saccharomyces cerevisiae
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
-
390847
Saccharomyces cerevisiae
?
Other publictions for EC 1.3.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745462
Kaneshiro
Sterols of Saccharomyces cere ...
Pneumocystis carinii, Pneumocystis carinii B80
J. Eukaryot. Microbiol.
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298-306
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684606
He
Ergosterol production from mol ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae YEH56(pHXA42)
Appl. Microbiol. Biotechnol.
75
55-60
2007
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654205
Visbal
S-adenosyl-L-methionine inhibi ...
Paracoccidioides brasiliensis
Antimicrob. Agents Chemother.
47
2966-2970
2003
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390848
Zweytick
Biochemical characterization a ...
Saccharomyces cerevisiae
FEBS Lett.
470
83-87
2000
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657077
Salimova
-
Sterol mutants of Chlamydomona ...
Chlamydomonas reinhardtii, Chlamydomonas reinhardtii arg7cw15
Plant Physiol. Biochem.
37
241-249
1999
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1
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390847
Pierce
Azasterol inhibitors in yeast. ...
Saccharomyces cerevisiae
Can. J. Biochem.
57
201-208
1979
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1
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1
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390846
Pierce
Azasterol inhibitors in yeast. ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
529
429-437
1978
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390845
Neal
Sterol 24(28) methylene reduct ...
Saccharomyces cerevisiae
J. Bacteriol.
129
1375-1378
1977
1
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654951
Jarman
-
Biosynthesis of steroids and t ...
Saccharomyces cerevisiae
Bioorg. Chem.
4
202-211
1975
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2
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