BRENDA - Enzyme Database
show all sequences of 1.3.1.70

Sterols regulate development and gene expression in Arabidopsis

He, J.X.; Fujioka, S.; Li, T.C.; Kang, S.G.; Seto, H.; Takatsuto, S.; Yoshida, S.; Jang, J.C.; Plant Physiol. 131, 1258-1269 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
brassicasterols have regulatory function in the plants concerning growth and development, the enzyme is up-regulated by several growth-promoting hormones including brassinolide and auxin, overview
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
mutational blockage of the enzyme leads to accumulation of atypical products from substrate 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol in vivo, i.e. 5alpha-cholesta-8,14-dien-3beta-ol, (24R)-5alpha-ergosta-8,14-dien-3beta-ol, and (24R)-5alpha-stigmasta-8,14-dien-3beta-ol
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
fenpropimorph
impairs cell expansion and affects gene expression in vivo, the effects cannot be reversed by addition of exogenous brassicasterols, changes sterol composition, phenotype is similar to fk-mutant plants, molecular mechanism
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH + H+
Arabidopsis thaliana
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by enzyme FK
4alpha-methylfecosterol + NADP+
-
-
-
isofucosterol + NADPH
Arabidopsis thaliana
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by bifunctinal enzyme DIM1/CBB1/DWF1
sitosterol + NADP+
-
-
-
additional information
Arabidopsis thaliana
brassicasterols have regulatory function in the plants concerning growth and development, the enzyme is up-regulated by several growth-promoting hormones including brassinolide and auxin, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
gene fk, enzyme FK, and bifunctinal enzyme DIM1/CBB1/DWF1
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
fk gene expression pattern in wild-type and mutant strains, regulatory effects of sterols, overview
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH + H+
-
657046
Arabidopsis thaliana
4alpha-methylfecosterol + NADP+
-
-
-
-
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH + H+
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by enzyme FK
657046
Arabidopsis thaliana
4alpha-methylfecosterol + NADP+
-
-
-
-
isofucosterol + NADPH
-
657046
Arabidopsis thaliana
sitosterol + NADP+
-
-
-
-
isofucosterol + NADPH
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by bifunctinal enzyme DIM1/CBB1/DWF1
657046
Arabidopsis thaliana
sitosterol + NADP+
-
-
-
-
additional information
brassicasterols have regulatory function in the plants concerning growth and development, the enzyme is up-regulated by several growth-promoting hormones including brassinolide and auxin, overview
657046
Arabidopsis thaliana
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
brassicasterols have regulatory function in the plants concerning growth and development, the enzyme is up-regulated by several growth-promoting hormones including brassinolide and auxin, overview
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
mutational blockage of the enzyme leads to accumulation of atypical products from substrate 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol in vivo, i.e. 5alpha-cholesta-8,14-dien-3beta-ol, (24R)-5alpha-ergosta-8,14-dien-3beta-ol, and (24R)-5alpha-stigmasta-8,14-dien-3beta-ol
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
fenpropimorph
impairs cell expansion and affects gene expression in vivo, the effects cannot be reversed by addition of exogenous brassicasterols, changes sterol composition, phenotype is similar to fk-mutant plants, molecular mechanism
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH + H+
Arabidopsis thaliana
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by enzyme FK
4alpha-methylfecosterol + NADP+
-
-
-
isofucosterol + NADPH
Arabidopsis thaliana
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by bifunctinal enzyme DIM1/CBB1/DWF1
sitosterol + NADP+
-
-
-
additional information
Arabidopsis thaliana
brassicasterols have regulatory function in the plants concerning growth and development, the enzyme is up-regulated by several growth-promoting hormones including brassinolide and auxin, overview
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
fk gene expression pattern in wild-type and mutant strains, regulatory effects of sterols, overview
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH + H+
-
657046
Arabidopsis thaliana
4alpha-methylfecosterol + NADP+
-
-
-
-
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH + H+
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by enzyme FK
657046
Arabidopsis thaliana
4alpha-methylfecosterol + NADP+
-
-
-
-
isofucosterol + NADPH
-
657046
Arabidopsis thaliana
sitosterol + NADP+
-
-
-
-
isofucosterol + NADPH
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by bifunctinal enzyme DIM1/CBB1/DWF1
657046
Arabidopsis thaliana
sitosterol + NADP+
-
-
-
-
additional information
brassicasterols have regulatory function in the plants concerning growth and development, the enzyme is up-regulated by several growth-promoting hormones including brassinolide and auxin, overview
657046
Arabidopsis thaliana
?
-
-
-
-
Other publictions for EC 1.3.1.70
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746161
Huang
Fackel interacts with gibbere ...
Arabidopsis thaliana
Planta
245
939-950
2017
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2
2
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744648
Bartoli
Impaired cell proliferation i ...
Mus musculus
Cell Cycle
15
2164-2173
2016
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1
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1
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2
2
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745882
Li
Structure of an integral memb ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Nature
517
104-107
2015
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1
1
11
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2
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6
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1
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8
1
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1
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1
11
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1
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8
1
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3
3
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725903
Liu
A sterol C-14 reductase encode ...
Fusarium graminearum
Microbiology
157
1665-1675
2011
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3
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5
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2
2
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711302
Schiavoni
Activation of TM7SF2 promoter ...
Homo sapiens
Biochim. Biophys. Acta
1801
587-592
2010
-
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1
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1
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1
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1
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2
1
1
2
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713446
Wang
Gonadotropin-regulated express ...
Oryctolagus cuniculus
Prostaglandins Other Lipid Mediat.
92
25-32
2010
-
-
1
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1
1
1
1
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725863
Hata
Inhibition of ergosterol synth ...
Candida albicans
Med. Mycol.
48
613-621
2010
-
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1
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2
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5
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723885
Liang
2-Amino-nonyl-6-methoxyl-tetra ...
Candida albicans
Acta Pharmacol. Sin.
30
1709
2009
-
-
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1
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4
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1
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1
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688770
Shah Alam Bhuiyan
Synthetically lethal interacti ...
Saccharomyces cerevisiae
Lipids
42
69-76
2007
-
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-
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2
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672378
Bennati
Sterol dependent regulation of ...
Homo sapiens
Biochim. Biophys. Acta
1761
677-685
2006
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-
1
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2
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2
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3
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22
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4
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1
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2
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22
-
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4
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655352
Yamashita
Gonadotropin-induced DELTA14-r ...
Sus scrofa
Endocrinology
146
186-194
2005
1
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1
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2
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2
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4
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2
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4
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2
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654166
Waterham
Autosomal recessive HEM/Greenb ...
Homo sapiens
Am. J. Hum. Genet.
72
1013-1017
2003
-
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1
-
1
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2
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1
2
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4
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1
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3
1
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1
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2
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1
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1
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657046
He
Sterols regulate development a ...
Arabidopsis thaliana
Plant Physiol.
131
1258-1269
2003
1
-
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1
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1
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3
-
3
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1
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5
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1
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1
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1
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1
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1
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5
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288492
Roberti
Cloning and expression of ster ...
Bos taurus, Homo sapiens
Eur. J. Biochem.
269
283-290
2002
-
-
2
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-
6
-
2
4
-
7
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1
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3
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12
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1
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1
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2
2
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6
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4
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1
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3
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12
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1
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654204
Jia
Candida albicans sterol C-14 r ...
Candida albicans
Antimicrob. Agents Chemother.
46
947-957
2002
-
1
1
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1
-
13
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1
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5
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1
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1
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13
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656304
Prakash
Genes encoding chimeras of Neu ...
Homo sapiens, Neurospora crassa
J. Biosci.
27
105-112
2002
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2
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3
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2
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8
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1
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1
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656798
Prakash
The sterol C-14 reductase enco ...
Neurospora crassa
Mol. Genet. Genomics
266
787-795
2002
-
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1
-
38
-
1
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1
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3
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1
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1
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2
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1
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1
1
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38
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1
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1
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2
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288491
Kim
Cholesterol biosynthesis from ...
Rattus norvegicus
Biochim. Biophys. Acta
1259
39-48
1995
4
-
-
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2
7
1
2
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2
3
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3
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1
1
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3
1
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5
1
1
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4
4
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1
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4
7
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3
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1
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1
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5
1
1
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288494
Lorenz
Cloning, sequencing, and disru ...
Saccharomyces cerevisiae
DNA Cell Biol.
11
685-692
1992
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1
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288493
Taton
Microsomal DELTA8,14-sterol DE ...
Embryophyta, Zea mays
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1989
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28
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16
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28
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16
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288495
Baloch
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Inhibition of sterol DELTA8 -> ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae NCYC 739, Ustilago maydis
Phytochemistry
26
663-668
1987
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288490
Paik
Microsomal enzymes of choleste ...
Rattus norvegicus
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259
13413-13423
1984
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1
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3
12
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15
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288489
Bottema
DELTA14-Sterol reductase in Sa ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae 3701B
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531
301-307
1978
2
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