Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxylic acid diol + NAD+ | Pseudomonas putida | - |
catechol + NADH | - |
? | |
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ | Pseudomonas putida | no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ | Pseudomonas putida BG1 | no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-p-toluate diol + NAD+ | Pseudomonas putida | metabolism of toluene | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-p-toluate diol + NAD+ | Pseudomonas putida BG1 | metabolism of toluene | 4-methylcatechol + NADH + H+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
harboring TOL plasmid | - |
Pseudomonas putida BG1 | - |
harboring TOL plasmid | - |
Purification (Comment) | Organism |
---|---|
French press, DEAE-cellulose column, dialysis, Red A column, ultrafiltration, purified 38fold | Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD(P)+ = 4-methylcatechol + NAD(P)H + H+ + CO2 | enzyme utilizes NAD+ but not NAD(P) | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.067 | - |
with plasmid pDTG501 and carbon source p-toluate | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxycyclohexa-3,5-diene 1-carboxylate + NAD+ | - |
Pseudomonas putida | catechol + NADH | - |
? | |
1,2-dihydroxycyclohexa-3,5-diene 1-carboxylate + NAD+ | - |
Pseudomonas putida BG1 | catechol + NADH | - |
? | |
carboxylic acid diol + NAD+ | - |
Pseudomonas putida | catechol + NADH | - |
? | |
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ | - |
Pseudomonas putida | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ | no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate | Pseudomonas putida | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ | - |
Pseudomonas putida BG1 | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+ | no activity observed when cis-p-toluene dihydrodiol, cis-naphthalenedihydrodiol, or the methyl ester of cis-p-toluate diol used as a substrate | Pseudomonas putida BG1 | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-p-toluate diol + NAD+ | - |
Pseudomonas putida | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-p-toluate diol + NAD+ | metabolism of toluene | Pseudomonas putida | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-p-toluate diol + NAD+ | - |
Pseudomonas putida BG1 | 4-methylcatechol + NADH + H+ + CO2 | - |
? | |
cis-p-toluate diol + NAD+ | metabolism of toluene | Pseudomonas putida BG1 | 4-methylcatechol + NADH + H+ + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas putida |