Cloned (Comment) | Organism |
---|---|
gene bphB, overexpression of N-terminally His-tagged BphB in Escherichia coli strain BL21 (DE3) | Comamonas testosteroni |
Crystallization (Comment) | Organism |
---|---|
purified recombinant detagged BphB, sitting drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 20 mM HEPES, pH 8.0, 10% glycerol, and 300 mM NaCl, are mixed with 0.001 ml of reservoir solution containing 0.2 M sodium malonate, pH 6.0, and 20% PEG 3350, 20% glycerol is used as cryoprotectant, X-ray diffractiuon structure determination and analysis at 2.8 A resolution | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29400 | - |
x * 29400, about | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | Q46381 | gene bphB | - |
Comamonas testosteroni B-356 | Q46381 | gene bphB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged BphB from Escherichia coli strain BL21 (DE3) to homogeneity by niockel affinity chromatography, the His-tag is cleaved off followed by ultrafiltration and gel filtration | Comamonas testosteroni |
Subunits | Comment | Organism |
---|---|---|
? | x * 29400, about | Comamonas testosteroni |
Synonyms | Comment | Organism |
---|---|---|
biphenyl dihydrodiol dehydrogenase | - |
Comamonas testosteroni |
BphB | - |
Comamonas testosteroni |
BphBB356 | - |
Comamonas testosteroni |
cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase | - |
Comamonas testosteroni |
More | the enzyme belongs to the short-chain alcohol dehydrogenase/reductase, SDR, family | Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Comamonas testosteroni |