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Literature summary for 1.3.1.56 extracted from

  • Seeger, M.; Gonzalez, M.; Camara, B.; Munoz, L.; Ponce, E.; Mejias, L.; Mascayano, C.; Vasquez, Y.; Sepulveda-Boza, S.
    Biotransformation of natural and synthetic isoflavonoids by two recombinant microbial enzymes (2003), Appl. Environ. Microbiol., 69, 5045-5050.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.56

Cloned(Commentary)

Cloned (Comment) Organism
functional coexpression of enzymes BphB and BphA in Escherichia coli, gene construct bphA1A2A3A4B in the plasmid vector Burkholderia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-hydroxy-8-methylisoflavone + 2 NAD+ + 2 H2O Burkholderia sp. 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells 7,2',3'-trihydroxy-8-methylisoflavone + 2 NADH + 2 H+
-
 ?
7-hydroxy-8-methylisoflavone + 2 NAD+ + 2 H2O Burkholderia sp. LB400 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells 7,2',3'-trihydroxy-8-methylisoflavone + 2 NADH + 2 H+
-
 ?
7-hydroxyisoflavone + 2 NAD+ + 2 H2O Burkholderia sp. 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells 7,3',4'-trihydroxyisoflavone + 2 NADH + 2 H+
-
 ?
7-hydroxyisoflavone + 2 NAD+ + 2 H2O Burkholderia sp. LB400 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells 7,3',4'-trihydroxyisoflavone + 2 NADH + 2 H+
-
 ?
additional information Burkholderia sp. biotransformation of natural and synthetic isoflavonoids by the recombinant enzyme, overview ?
-
 ?
additional information Burkholderia sp. LB400 biotransformation of natural and synthetic isoflavonoids by the recombinant enzyme, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
 enzyme BphB
-
Burkholderia sp. LB400
-
 enzyme BphB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,2',3'-trihydroxy-8-methyl-2',3'-dihydro-isoflavone + NAD+ recombinant enzyme Burkholderia sp. 7,2',3'-trihydroxy-8-methylisoflavone + NADH
-
 ?
7,2',3'-trihydroxy-8-methyl-2',3'-dihydro-isoflavone + NAD+ recombinant enzyme Burkholderia sp. LB400 7,2',3'-trihydroxy-8-methylisoflavone + NADH
-
 ?
7,3',4'-trihydroxy-3',4'-dihydro-isoflavone + NAD+ recombinant enzyme Burkholderia sp. 7,3',4'-trihydroxyisoflavone + NADH
-
 ?
7-hydroxy-8-methylisoflavone + 2 NAD+ + 2 H2O 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells Burkholderia sp. 7,2',3'-trihydroxy-8-methylisoflavone + 2 NADH + 2 H+
-
 ?
7-hydroxy-8-methylisoflavone + 2 NAD+ + 2 H2O 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells Burkholderia sp. LB400 7,2',3'-trihydroxy-8-methylisoflavone + 2 NADH + 2 H+
-
 ?
7-hydroxyisoflavone + 2 NAD+ + 2 H2O 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells Burkholderia sp. 7,3',4'-trihydroxyisoflavone + 2 NADH + 2 H+
-
 ?
7-hydroxyisoflavone + 2 NAD+ + 2 H2O 2-step biotransformation in cooperation with biphenyl-2,3-dioxygenase, BphA, catalyzing the first step and BphB the second step, recombinant enzymes in Escherichia coli cells Burkholderia sp. LB400 7,3',4'-trihydroxyisoflavone + 2 NADH + 2 H+
-
 ?
additional information biotransformation of natural and synthetic isoflavonoids by the recombinant enzyme, overview Burkholderia sp. ?
-
 ?
additional information enzyme shows a broad substrate specificity Burkholderia sp. ?
-
 ?
additional information biotransformation of natural and synthetic isoflavonoids by the recombinant enzyme, overview Burkholderia sp. LB400 ?
-
 ?
additional information enzyme shows a broad substrate specificity Burkholderia sp. LB400 ?
-
 ?

Synonyms

Synonyms Comment Organism
biphenyl-2,3-dihydrodiol 2,3-dehydrogenase
-
 Burkholderia sp.
BphB
-
 Burkholderia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at, cell culture temperature for in vivo experiments Burkholderia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at, medium for in vivo experiments Burkholderia sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Burkholderia sp.