Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.56 extracted from

  • Vedadi, M.; Barriault, D.; Sylvestre, M; Powlowski, J.
    Active site residues of cis-2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase from Comamonas testosteroni strain B-356 (2000), Biochemistry, 39, 5028-5034.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00024
-
NAD(P)+ at pH 9 and 25°C Comamonas testosteroni
0.0031
-
cis-2,3-dihydro-2,3-dihydroxybiphenyl at pH 9 and 25°C Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29400
-
4 * 29400, electrospray mass spectrometry Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD(P)+ Comamonas testosteroni second step of the metabolic pathway for the bacterial degradation of biphenyl 2,3-dihydroxybiphenyl + NAD(P)H
-
?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose chromatography, yield about 50 mg of enzyme/l of cell culture Comamonas testosteroni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.4
-
after purification at 25°C and pH 9 Comamonas testosteroni

Storage Stability

Storage Stability Organism
-20°C stable in 50% glycerol for months Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD(P)+
-
Comamonas testosteroni 2,3-dihydroxybiphenyl + NAD(P)H
-
?
cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD(P)+ second step of the metabolic pathway for the bacterial degradation of biphenyl Comamonas testosteroni 2,3-dihydroxybiphenyl + NAD(P)H
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 29400, electrospray mass spectrometry Comamonas testosteroni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
Comamonas testosteroni