KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00024 | - |
NAD(P)+ | at pH 9 and 25°C | Comamonas testosteroni | |
0.0031 | - |
cis-2,3-dihydro-2,3-dihydroxybiphenyl | at pH 9 and 25°C | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29400 | - |
4 * 29400, electrospray mass spectrometry | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD(P)+ | Comamonas testosteroni | second step of the metabolic pathway for the bacterial degradation of biphenyl | 2,3-dihydroxybiphenyl + NAD(P)H | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose chromatography, yield about 50 mg of enzyme/l of cell culture | Comamonas testosteroni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.4 | - |
after purification at 25°C and pH 9 | Comamonas testosteroni |
Storage Stability | Organism |
---|---|
-20°C stable in 50% glycerol for months | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD(P)+ | - |
Comamonas testosteroni | 2,3-dihydroxybiphenyl + NAD(P)H | - |
? | |
cis-2,3-dihydro-2,3-dihydroxybiphenyl + NAD(P)+ | second step of the metabolic pathway for the bacterial degradation of biphenyl | Comamonas testosteroni | 2,3-dihydroxybiphenyl + NAD(P)H | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 29400, electrospray mass spectrometry | Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Comamonas testosteroni |