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Literature summary for 1.3.1.5 extracted from

  • Schabort, J.C.; Potgieter, D.J.J.
    Cucurbitacin B DELTA23-reductase from Cucurbita maxima. II. Cofactor requirements, enzyme kinetics, substrate specificity and other characteristics (1968), Biochim. Biophys. Acta, 151, 47-53.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0604
-
 NADH
-
 Cucurbita maxima

Organism

Organism UniProt Comment Textmining
Cucurbita maxima
-
 var. Green Hubbard
-

Reaction

Reaction Comment Organism Reaction ID
23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+ plant terpenoid Cucurbita maxima
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cucurbitacin A + NAD(P)H very low activity Cucurbita maxima 23,24-dihydrocucurbitacin A + NAD(P)+
-
 ?
cucurbitacin B + NAD(P)H + H+ NADPH better electron donor than NADH, reduction is fairly irreversible, approx. 75% of activity with cucurbitacin E beta-D-glucoside Cucurbita maxima 23,24-dihydrocucurbitacin B + NAD(P)+
-
 ir
cucurbitacin B + NADH + H+
-
 Cucurbita maxima 23,24-dihydrocucurbitacin B + NAD+
-
 r
cucurbitacin C + NAD(P)H very low activity Cucurbita maxima 23,24-dihydrocucurbitacin C + NAD(P)+
-
 ?
cucurbitacin D + NAD(P)H low activity Cucurbita maxima 23,24-dihydrocucurbitacin D + NAD(P)+
-
 ?
cucurbitacin E + NAD(P)H low activity Cucurbita maxima 23,24-dihydrocucurbitacin E + NAD(P)+
-
 ?
cucurbitacin E beta-D-glucoside + NAD(P)H best substrate Cucurbita maxima 23,24-dihydrocucurbitacin E beta-D-glucoside + NAD(P)+
-
 ?
cucurbitacin F + NAD(P)H very low activity Cucurbita maxima 23,24-dihydrocucurbitacin F + NAD(P)+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
 Cucurbita maxima