Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Alcaligenes faecalis | |
PCMB | - |
Alcaligenes faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
(+)-cis-3,4-dihydrophenanthrene-3,4-diol | - |
Alcaligenes faecalis | |
0.058 | - |
NAD+ | - |
Alcaligenes faecalis | |
0.63 | - |
NADP+ | - |
Alcaligenes faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
4 * 27500, SDS-PAGE | Alcaligenes faecalis |
100000 | - |
gel filtration | Alcaligenes faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ | Alcaligenes faecalis | enzyme catalyzes an early step in the degradation of phenanthrene | phenanthrene-3,4-diol + NADH | - |
? | |
(+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ | Alcaligenes faecalis AFK2 | enzyme catalyzes an early step in the degradation of phenanthrene | phenanthrene-3,4-diol + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Alcaligenes faecalis AFK2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Alcaligenes faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70.7 | - |
- |
Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ | - |
Alcaligenes faecalis | phenanthrene-3,4-diol + NADH | - |
? | |
(+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ | enzyme catalyzes an early step in the degradation of phenanthrene | Alcaligenes faecalis | phenanthrene-3,4-diol + NADH | - |
? | |
(+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ | - |
Alcaligenes faecalis AFK2 | phenanthrene-3,4-diol + NADH | - |
? | |
(+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ | enzyme catalyzes an early step in the degradation of phenanthrene | Alcaligenes faecalis AFK2 | phenanthrene-3,4-diol + NADH | - |
? | |
(+)-cis-3,4-dihydrophenanthrene-3,4-diol + NADP+ | - |
Alcaligenes faecalis | phenanthrene-3,4-diol + NADPH + H+ | - |
r | |
(+)-cis-3,4-dihydrophenanthrene-3,4-diol + NADP+ | - |
Alcaligenes faecalis AFK2 | phenanthrene-3,4-diol + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 27500, SDS-PAGE | Alcaligenes faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Alcaligenes faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Alcaligenes faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | electron acceptor | Alcaligenes faecalis | |
NADP+ | electron acceptor | Alcaligenes faecalis |