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Literature summary for 1.3.1.48 extracted from
- Purification and characterization of a 15-ketoprostaglandin delta 13-reductase from bovine lung (1979), Biochim. Biophys. Acta, 574, 136-145.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Bos taurus |  |
| p-chloromercuribenzoate | 0.1 mM, complete inhibition | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | 0.009 | NADPH | - |
Bos taurus | |
| 0.088 | 0.094 | NADH | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lung | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.067 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -4°C, 0.1 M sodium phosphate, pH 7.4, 1 mM EDTA, 1 mM mercaptoethanol, stable for more than 1 month | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H | no substrate: prostaglandin E1 | Bos taurus | (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ | - |
ir | |
| 15-ketoprostaglandin E + NAD(P)H | - |
Bos taurus | ? | - |
? | |
| 15-ketoprostaglandin E2 + NADH + H+ | - |
Bos taurus | 13,14-dihydro-15-ketoprostaglandin E2 + NAD+ | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | 1 | 15-ketoprostaglandin E | - |
Bos taurus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD(P)H | bovine lung: enzyme can use both NADPH and NADH | Bos taurus | |
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Release 2023.1 (January 2023)
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