Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cibacron blue 3 G-A | competitive inhibition vs. NADH, noncompetitive vs. 15-keto-prostaglandin E2 | Homo sapiens | |
NADPH | competitive inhibition vs. NADH, noncompetitive vs. 15-keto-prostaglandin E2 | Homo sapiens | |
p-chloromercuribenzoate | 0.0001 mM, 85% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
15-ketoprostaglandin E1 | - |
Homo sapiens | |
0.0075 | - |
15-ketoprostaglandin A1 | - |
Homo sapiens | |
0.0108 | - |
15-ketoprostaglandin F2alpha | - |
Homo sapiens | |
0.0112 | - |
15-ketoprostaglandin B1 | - |
Homo sapiens | |
0.0127 | - |
15-ketoprostaglandin E2 | - |
Homo sapiens | |
0.032 | - |
NADH | - |
Homo sapiens | |
0.063 | - |
nicotinamide hypoxanthine dinucleotide | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.425 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol v/v, 5 mM potassium phosphate, 1 mM EDTA, pH 7.0, stable for at least 6 months | Homo sapiens |
4°C, stable for several weeks in absence of glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H | no substrates: 15-hydroxyprostaglandins, progesterone, cortisone, orotic acid, fumaric acid | Homo sapiens | (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ | - |
ir | |
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H | i.e. 15-ketoprostaglandin E2 | Homo sapiens | 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+ | - |
? | |
15-ketoprostaglandin A1 + NAD(P)H | - |
Homo sapiens | ? | - |
? | |
15-ketoprostaglandin B1 + NAD(P)H | - |
Homo sapiens | ? | - |
? | |
15-ketoprostaglandin E1 + NADH + H+ | - |
Homo sapiens | 13,14-dihydro-15-keto-prostaglandin E1 + NAD+ | - |
r | |
15-ketoprostaglandin E1 + NADPH | - |
Homo sapiens | ? | - |
? | |
15-ketoprostaglandin E1 + reduced nicotinamide hypoxanthine dinucleotide + H+ | - |
Homo sapiens | 13,14-dihydro-15-keto-prostaglandin E1 + nicotinamide hypoxanthine dinucleotide | - |
r | |
15-ketoprostaglandin F2alpha + NADPH | - |
Homo sapiens | ? | - |
? | |
9,11-dihydroxy-15-oxoprosta-5,13-dien-1-oic acid + NAD(P)H | i.e. 15-ketoprostaglandin F2alpha | Homo sapiens | 9,11-dihydroxy-15-oxoprosta-5-en-1-oic aicd + NAD(P)+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Homo sapiens | |
NADH | human placenta: enzyme can use only NADH | Homo sapiens | |
nicotinamide hypoxanthine dinucleotide | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
NADPH | - |
Homo sapiens | |
0.0000125 | - |
Cibacron Blue 3G-A | - |
Homo sapiens |