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Literature summary for 1.3.1.48 extracted from

  • Jarabak, J.
    Isolation and properties of a 15-ketoprostaglandin delta 13-reductase from human placenta (1982), Methods Enzymol., 86, 163-167.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue 3 G-A competitive inhibition vs. NADH, noncompetitive vs. 15-keto-prostaglandin E2 Homo sapiens
NADPH competitive inhibition vs. NADH, noncompetitive vs. 15-keto-prostaglandin E2 Homo sapiens
p-chloromercuribenzoate 0.0001 mM, 85% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
15-ketoprostaglandin E1
-
Homo sapiens
0.0075
-
15-ketoprostaglandin A1
-
Homo sapiens
0.0108
-
15-ketoprostaglandin F2alpha
-
Homo sapiens
0.0112
-
15-ketoprostaglandin B1
-
Homo sapiens
0.0127
-
15-ketoprostaglandin E2
-
Homo sapiens
0.032
-
NADH
-
Homo sapiens
0.063
-
nicotinamide hypoxanthine dinucleotide
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.425
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol v/v, 5 mM potassium phosphate, 1 mM EDTA, pH 7.0, stable for at least 6 months Homo sapiens
4°C, stable for several weeks in absence of glycerol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H no substrates: 15-hydroxyprostaglandins, progesterone, cortisone, orotic acid, fumaric acid Homo sapiens (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
ir
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H i.e. 15-ketoprostaglandin E2 Homo sapiens 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
?
15-ketoprostaglandin A1 + NAD(P)H
-
Homo sapiens ?
-
?
15-ketoprostaglandin B1 + NAD(P)H
-
Homo sapiens ?
-
?
15-ketoprostaglandin E1 + NADH + H+
-
Homo sapiens 13,14-dihydro-15-keto-prostaglandin E1 + NAD+
-
r
15-ketoprostaglandin E1 + NADPH
-
Homo sapiens ?
-
?
15-ketoprostaglandin E1 + reduced nicotinamide hypoxanthine dinucleotide + H+
-
Homo sapiens 13,14-dihydro-15-keto-prostaglandin E1 + nicotinamide hypoxanthine dinucleotide
-
r
15-ketoprostaglandin F2alpha + NADPH
-
Homo sapiens ?
-
?
9,11-dihydroxy-15-oxoprosta-5,13-dien-1-oic acid + NAD(P)H i.e. 15-ketoprostaglandin F2alpha Homo sapiens 9,11-dihydroxy-15-oxoprosta-5-en-1-oic aicd + NAD(P)+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
Homo sapiens
NADH human placenta: enzyme can use only NADH Homo sapiens
nicotinamide hypoxanthine dinucleotide
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001
-
NADPH
-
Homo sapiens
0.0000125
-
Cibacron Blue 3G-A
-
Homo sapiens