BRENDA - Enzyme Database
show all sequences of 1.3.1.47

alpha-Santonin 1,2-reductase and its role in the formation of dihydrosantonin and lumisantonin by Pseudomonas cichorii S

Naik, U.; Mavuinkurve, S.; Can. J. Microbiol. 33, 658-662 (1987)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
NADH
-
Pseudomonas cichorii
0.067
-
alpha-santonin
-
Pseudomonas cichorii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-santonin + NAD(P)H
Pseudomonas cichorii
degradative pathway of alpha-santonin
1,2-dihydrosantonin + NAD(P)+
-
Pseudomonas cichorii
-
alpha-santonin + NAD(P)H
Pseudomonas cichorii S
degradative pathway of alpha-santonin
1,2-dihydrosantonin + NAD(P)+
-
Pseudomonas cichorii S
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas cichorii
-
capable of using santonin as sole source of carbon and energy
-
Pseudomonas cichorii S
-
capable of using santonin as sole source of carbon and energy
-
Reaction
Reaction
Commentary
Organism
1,2-dihydrosantonin + NAD(P)+ = alpha-santonin + NAD(P)H + H+
proposed mechanism
Pseudomonas cichorii
Storage Stability
Storage Stability
Organism
8-10°C, activity reduced
Pseudomonas cichorii
below 0°C, 50% loss of activity after 48 h
Pseudomonas cichorii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-santonin + NAD(P)H
-
390819
Pseudomonas cichorii
1,2-dihydrosantonin + NAD(P)+
-
390819
Pseudomonas cichorii
?
alpha-santonin + NAD(P)H
degradative pathway of alpha-santonin
390819
Pseudomonas cichorii
1,2-dihydrosantonin + NAD(P)+
-
390819
Pseudomonas cichorii
-
alpha-santonin + NAD(P)H
-
390819
Pseudomonas cichorii S
1,2-dihydrosantonin + NAD(P)+
-
390819
Pseudomonas cichorii S
?
alpha-santonin + NAD(P)H
degradative pathway of alpha-santonin
390819
Pseudomonas cichorii S
1,2-dihydrosantonin + NAD(P)+
-
390819
Pseudomonas cichorii S
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pseudomonas cichorii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8
no change in activity
Pseudomonas cichorii
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas cichorii
NADPH
2fold higher reaction velocity as with NADH
Pseudomonas cichorii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas cichorii
NADPH
2fold higher reaction velocity as with NADH
Pseudomonas cichorii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
NADH
-
Pseudomonas cichorii
0.067
-
alpha-santonin
-
Pseudomonas cichorii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-santonin + NAD(P)H
Pseudomonas cichorii
degradative pathway of alpha-santonin
1,2-dihydrosantonin + NAD(P)+
-
Pseudomonas cichorii
-
alpha-santonin + NAD(P)H
Pseudomonas cichorii S
degradative pathway of alpha-santonin
1,2-dihydrosantonin + NAD(P)+
-
Pseudomonas cichorii S
-
Storage Stability (protein specific)
Storage Stability
Organism
8-10°C, activity reduced
Pseudomonas cichorii
below 0°C, 50% loss of activity after 48 h
Pseudomonas cichorii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-santonin + NAD(P)H
-
390819
Pseudomonas cichorii
1,2-dihydrosantonin + NAD(P)+
-
390819
Pseudomonas cichorii
?
alpha-santonin + NAD(P)H
degradative pathway of alpha-santonin
390819
Pseudomonas cichorii
1,2-dihydrosantonin + NAD(P)+
-
390819
Pseudomonas cichorii
-
alpha-santonin + NAD(P)H
-
390819
Pseudomonas cichorii S
1,2-dihydrosantonin + NAD(P)+
-
390819
Pseudomonas cichorii S
?
alpha-santonin + NAD(P)H
degradative pathway of alpha-santonin
390819
Pseudomonas cichorii S
1,2-dihydrosantonin + NAD(P)+
-
390819
Pseudomonas cichorii S
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Pseudomonas cichorii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8
no change in activity
Pseudomonas cichorii
Other publictions for EC 1.3.1.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
390819
Naik
alpha-Santonin 1,2-reductase a ...
Pseudomonas cichorii, Pseudomonas cichorii S
Can. J. Microbiol.
33
658-662
1987
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4
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1
1
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