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Literature summary for 1.3.1.47 extracted from

  • Naik, U.; Mavuinkurve, S.
    alpha-Santonin 1,2-reductase and its role in the formation of dihydrosantonin and lumisantonin by Pseudomonas cichorii S (1987), Can. J. Microbiol., 33, 658-662.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
NADH
-
Pseudomonas cichorii
0.067
-
alpha-santonin
-
Pseudomonas cichorii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-santonin + NAD(P)H Pseudomonas cichorii degradative pathway of alpha-santonin 1,2-dihydrosantonin + NAD(P)+
-
?
alpha-santonin + NAD(P)H Pseudomonas cichorii S degradative pathway of alpha-santonin 1,2-dihydrosantonin + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas cichorii
-
capable of using santonin as sole source of carbon and energy
-
Pseudomonas cichorii S
-
capable of using santonin as sole source of carbon and energy
-

Reaction

Reaction Comment Organism Reaction ID
1,2-dihydrosantonin + NAD(P)+ = alpha-santonin + NAD(P)H + H+ proposed mechanism Pseudomonas cichorii

Storage Stability

Storage Stability Organism
8-10°C, activity reduced Pseudomonas cichorii
below 0°C, 50% loss of activity after 48 h Pseudomonas cichorii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-santonin + NAD(P)H
-
Pseudomonas cichorii 1,2-dihydrosantonin + NAD(P)+
-
?
alpha-santonin + NAD(P)H degradative pathway of alpha-santonin Pseudomonas cichorii 1,2-dihydrosantonin + NAD(P)+
-
?
alpha-santonin + NAD(P)H
-
Pseudomonas cichorii S 1,2-dihydrosantonin + NAD(P)+
-
?
alpha-santonin + NAD(P)H degradative pathway of alpha-santonin Pseudomonas cichorii S 1,2-dihydrosantonin + NAD(P)+
-
?
alpha-santonin + NADH + H+
-
Pseudomonas cichorii 1,2-dihydrosantonin + NAD+
-
r
alpha-santonin + NADH + H+
-
Pseudomonas cichorii S 1,2-dihydrosantonin + NAD+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Pseudomonas cichorii

pH Range

pH Minimum pH Maximum Comment Organism
6 8 no change in activity Pseudomonas cichorii

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas cichorii
NADPH 2fold higher reaction velocity as with NADH Pseudomonas cichorii