KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
2'-hydroxyformononetin | - |
Cicer arietinum | |
0.035 | - |
NADPH | - |
Cicer arietinum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Cicer arietinum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-hydroxyformononetin + NADPH | Cicer arietinum | involved in medicarpin and maackiain biosynthesis | vestitone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cicer arietinum | - |
chickpea | - |
Oxidation Stability | Organism |
---|---|
complete inactivation without dithioerythritol in 6 h at 4°C | Cicer arietinum |
Purification (Comment) | Organism |
---|---|
partial | Cicer arietinum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Cicer arietinum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
- |
Cicer arietinum |
Storage Stability | Organism |
---|---|
-20°C, 20 mM potassium phosphate buffer, pH 7.5, 1 mM dithioerythritol, 5% glycerol | Cicer arietinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',7-dihydroxy-3',4'-methylenedioxyisoflavone + NADPH | - |
Cicer arietinum | 2',7-hydroxy-dihydro-3',4'-methylenedioxyisoflavone + NADP+ | - |
? | |
2'-hydroxyformononetin + NADPH | no reaction with isoflavones lacking 2'-hydroxy-group | Cicer arietinum | (3R)-vestitone + NADP+ | - |
? | |
2'-hydroxyformononetin + NADPH | involved in medicarpin and maackiain biosynthesis | Cicer arietinum | vestitone + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Cicer arietinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Cicer arietinum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | 25-40% of activity with NADPH | Cicer arietinum | |
NADPH | - |
Cicer arietinum |