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Literature summary for 1.3.1.44 extracted from
- Biochemical and structural characterization of the trans-enoyl-CoA reductase from Treponema denticola (2012), Biochemistry, 51, 6827-6837.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Treponema denticola |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure of tdTer is determined to 2.00 A | Treponema denticola |
| to 2.0 A resolution | Treponema denticola |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F295A | mutant shows kinetic behaviour relatively similar to wild-type toward substrates crotonyl-CoA and hexanoyl-CoA | Treponema denticola |
| I287A | mutant shows significant decreased kcat values compared to wild-type, mutant exhibit larger increases in catalytic efficiency on the longer hexanoyl-CoA substrate (versus the crotonyl-CoA substrate) of 100 and 17fold compared to that of the wild type (7fold) suggesting suggest these mutations may increase the accessibility of the longer acyl chain to the active site pocket. Mutant shows a much lower Ki (lauroyl-CoA) than wild-type | Treponema denticola |
| L276A/V277A | mutant shows significant decreased kcat values compared to wild-type | Treponema denticola |
| L276A/V277A/F295A | mutant shows significant decreased kcat values compared to wild-type, mutant exhibit larger increases in catalytic efficiency on the longer hexanoyl-CoA substrate (versus the crotonyl-CoA substrate) of 100 and 17fold compared to that of the wild type (7fold) suggesting suggest these mutations may increase the accessibility of the longer acyl chain to the active site pocket | Treponema denticola |
| L291A | mutant shows kinetic behaviour relatively similar to wild-type toward substrates crotonyl-CoA and hexanoyl-CoA | Treponema denticola |
| Y240F | Y240F mutation leads to a 5000fold decrease in catalytic efficiency compared to wild-type with no significant change in Km | Treponema denticola |
| Y370A | mutant shows kinetic behaviour relatively similar to wild-type toward substrates crotonyl-CoA and hexanoyl-CoA | Treponema denticola |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| butyryl-CoA | - |
Treponema denticola |  |
| hexanoyl-CoA | - |
Treponema denticola | |
| lauroyl-CoA | - |
Treponema denticola | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
trans-2-dodecenoyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.003 | - |
trans-2-dodecenoyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.0034 | - |
trans-2-hexenoyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.0034 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant I287A | Treponema denticola | |
| 0.004 | - |
trans-2-dodecenoyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.004 | - |
2-dodecenoyl-CoA | pH and temperature not specified in the publication, mutant I287A | Treponema denticola | |
| 0.0052 | - |
NADH | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.007 | - |
trans-2-hexenoyl-CoA | mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.007 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A | Treponema denticola | |
| 0.012 | - |
trans-2-hexenoyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.012 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, wild-type | Treponema denticola | |
| 0.015 | - |
trans-2-hexenoyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.015 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A/F295A | Treponema denticola | |
| 0.018 | - |
trans-2-hexenoyl-CoA | mutant Y370A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.018 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant Y370A | Treponema denticola | |
| 0.019 | - |
trans-2-hexenoyl-CoA | mutant F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.019 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant F295A | Treponema denticola | |
| 0.038 | - |
trans-2-hexenoyl-CoA | mutant L291A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.038 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant L291A | Treponema denticola | |
| 0.049 | - |
crotonyl-CoA | mutant Y240F, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.049 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant Y240F | Treponema denticola | |
| 0.06 | - |
crotonyl-CoA | mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.06 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A | Treponema denticola | |
| 0.07 | - |
crotonyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.07 | - |
crotonyl-CoA | pH and temperature not specified in the publication, wild-type | Treponema denticola | |
| 0.1 | - |
crotonyl-CoA | mutant F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.1 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant F295A | Treponema denticola | |
| 0.11 | - |
crotonyl-CoA | mutant L291A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.11 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant L291A | Treponema denticola | |
| 0.15 | - |
crotonyl-CoA | mutant Y370A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.15 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant Y370A | Treponema denticola | |
| 0.16 | - |
crotonyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.16 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant I287A | Treponema denticola | |
| 0.19 | - |
NADPH | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.21 | - |
crotonyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.21 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A/F295A | Treponema denticola | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Treponema denticola | Q73Q47 | - |
- |
| Treponema denticola ATCC 35405 | Q73Q47 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-2-hexanoyl-CoA + NADPH | - |
Treponema denticola | hexanoyl-CoA + NADP+ | - |
? | |
| (E)-2-hexanoyl-CoA + NADPH | - |
Treponema denticola ATCC 35405 | hexanoyl-CoA + NADP+ | - |
? | |
| crotonyl-CoA + NADH + H+ | - |
Treponema denticola | butyryl-CoA + NAD+ | - |
? | |
| crotonyl-CoA + NADH + H+ | - |
Treponema denticola ATCC 35405 | butyryl-CoA + NAD+ | - |
? | |
| crotonyl-CoA + NADPH | - |
Treponema denticola | butanoyl-CoA + NADP+ | - |
? | |
| crotonyl-CoA + NADPH | - |
Treponema denticola ATCC 35405 | butanoyl-CoA + NADP+ | - |
? | |
| crotonyl-CoA + NADPH + H+ | NADPH is a poor cosubstrate | Treponema denticola | butyryl-CoA + NADP+ | - |
? | |
| crotonyl-CoA + NADPH + H+ | NADPH is a poor cosubstrate | Treponema denticola ATCC 35405 | butyryl-CoA + NADP+ | - |
? | |
| trans-2-dodecenoyl-CoA + NADH + H+ | - |
Treponema denticola | dodecanoyl-CoA + NAD+ | - |
? | |
| trans-2-dodecenoyl-CoA + NADH + H+ | - |
Treponema denticola ATCC 35405 | dodecanoyl-CoA + NAD+ | - |
? | |
| trans-2-hexenoyl-CoA + NADH + H+ | - |
Treponema denticola | hexanoyl-CoA + NAD+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tde_0597 | - |
Treponema denticola |
| tdTer | - |
Treponema denticola |
| TER | - |
Treponema denticola |
| trans-enoyl-CoA reductase | - |
Treponema denticola |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.73 | - |
crotonyl-CoA | mutant Y240F, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.73 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant Y240F | Treponema denticola | |
| 6 | - |
trans-2-dodecenoyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 6 | - |
2-dodecenoyl-CoA | pH and temperature not specified in the publication, mutant I287A | Treponema denticola | |
| 7.1 | - |
crotonyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 7.1 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant I287A | Treponema denticola | |
| 15.3 | - |
trans-2-hexenoyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 15.3 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant I287A | Treponema denticola | |
| 15.6 | - |
crotonyl-CoA | mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 15.6 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A | Treponema denticola | |
| 18.9 | - |
crotonyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 18.9 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A/F295A | Treponema denticola | |
| 19 | - |
trans-2-hexenoyl-CoA | mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 19 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A | Treponema denticola | |
| 22.4 | - |
trans-2-hexenoyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 22.4 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A/F295A | Treponema denticola | |
| 24 | - |
trans-2-dodecenoyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 24 | - |
2-dodecenoyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A/F295A | Treponema denticola | |
| 37 | - |
crotonyl-CoA | mutant L291A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 37 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant L291A | Treponema denticola | |
| 48 | - |
trans-2-hexenoyl-CoA | mutant Y370A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 48 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant Y370A | Treponema denticola | |
| 50 | - |
trans-2-hexenoyl-CoA | mutant L291A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 50 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant L291A | Treponema denticola | |
| 64 | - |
trans-2-hexenoyl-CoA | mutant F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 64 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant F295A | Treponema denticola | |
| 73 | - |
crotonyl-CoA | mutant F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 73 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant F295A | Treponema denticola | |
| 90 | - |
trans-2-dodecenoyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 90 | - |
2-dodecenoyl-CoA | pH and temperature not specified in the publication, wild-type | Treponema denticola | |
| 91 | - |
crotonyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 91 | - |
crotonyl-CoA | pH and temperature not specified in the publication, wild-type | Treponema denticola | |
| 92 | - |
crotonyl-CoA | mutant Y370A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 92 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant Y370A | Treponema denticola | |
| 112 | - |
trans-2-hexenoyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 112 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, wild-type | Treponema denticola | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Treponema denticola | |
| NADPH | NADH is preferred over NADPH | Treponema denticola | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00003 | - |
lauroyl-CoA | pH and temperature not specified in the publication, 0.0001 mM inhibitor, mutant I287A | Treponema denticola | |
| 0.00006 | - |
lauroyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.00006 | - |
lauroyl-CoA | pH and temperature not specified in the publication, 0.00025 mM inhibitor, mutant I287A | Treponema denticola | |
| 0.0004 | - |
lauroyl-CoA | pH and temperature not specified in the publication, 0.0015 mM inhibitor, wild-type | Treponema denticola | |
| 0.0005 | - |
lauroyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.0005 | - |
lauroyl-CoA | pH and temperature not specified in the publication, 0.001 mM inhibitor, wild-type | Treponema denticola | |
| 0.001 | - |
lauroyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.003 | - |
lauroyl-CoA | pH and temperature not specified in the publication, 0.010 mM inhibitor, mutant I287A | Treponema denticola | |
| 0.01 | - |
lauroyl-CoA | pH and temperature not specified in the publication, 0.005 mM inhibitor, mutant I287A | Treponema denticola | |
| 0.05 | - |
hexanoyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.05 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, 0.05 mM inhibitor, wild-type | Treponema denticola | |
| 0.08 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, 0.25 mM inhibitor, wild-type | Treponema denticola | |
| 0.4 | - |
butyryl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 0.4 | - |
butyryl-CoA | pH and temperature not specified in the publication, 0.5 mM inhibitor, wild-type | Treponema denticola | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
crotonyl-CoA | mutant Y240F, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 15 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant Y240F | Treponema denticola | |
| 40 | - |
crotonyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 40 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant I287A | Treponema denticola | |
| 89 | - |
crotonyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 89 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A/F295A | Treponema denticola | |
| 260 | - |
crotonyl-CoA | mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 260 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A | Treponema denticola | |
| 300 | - |
crotonyl-CoA | mutant L291A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 300 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant L291A | Treponema denticola | |
| 390 | - |
NADPH | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 600 | - |
crotonyl-CoA | mutant Y370A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 600 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant Y370A | Treponema denticola | |
| 700 | - |
crotonyl-CoA | mutant F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 700 | - |
crotonyl-CoA | pH and temperature not specified in the publication, mutant F295A | Treponema denticola | |
| 1300 | - |
crotonyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 1300 | - |
trans-2-hexenoyl-CoA | mutant L291A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 1300 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant L291A | Treponema denticola | |
| 1300 | - |
crotonyl-CoA | pH and temperature not specified in the publication, wild-type | Treponema denticola | |
| 1500 | - |
trans-2-hexenoyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 1500 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A/F295A | Treponema denticola | |
| 2000 | - |
trans-2-dodecenoyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 2000 | - |
2-dodecenoyl-CoA | pH and temperature not specified in the publication, mutant I287A | Treponema denticola | |
| 2700 | - |
trans-2-hexenoyl-CoA | mutant Y370A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 2700 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant Y370A | Treponema denticola | |
| 3000 | - |
trans-2-hexenoyl-CoA | mutant L276A/V277A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 3000 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A | Treponema denticola | |
| 3400 | - |
trans-2-hexenoyl-CoA | mutant F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 3400 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant F295A | Treponema denticola | |
| 4500 | - |
trans-2-hexenoyl-CoA | mutant I287A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 4500 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, mutant I287A | Treponema denticola | |
| 9000 | - |
trans-2-hexenoyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 9000 | - |
hexanoyl-CoA | pH and temperature not specified in the publication, wild-type | Treponema denticola | |
| 16000 | - |
NADH | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 21000 | - |
trans-2-dodecenoyl-CoA | mutant L276A/V277A/F295A, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 21000 | - |
2-dodecenoyl-CoA | pH and temperature not specified in the publication, mutant L276A/V277A/F295A | Treponema denticola | |
| 30000 | - |
trans-2-dodecenoyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Treponema denticola | |
| 30000 | - |
2-dodecenoyl-CoA | pH and temperature not specified in the publication, wild-type | Treponema denticola | |
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