BRENDA - Enzyme Database
show all sequences of 1.3.1.42

Reductive and oxidative half-reactions of morphinone reductase from Pseudomonas putida M10: a kinetic and thermodynamic analysis

Craig, D.H.; Moody, P.C.; Bruce, N.C.; Scrutton, N.S.; Biochemistry 37, 7598-7607 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
cortisone
-
Pseudomonas putida
progesterone
-
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41100
-
2 * 41100
Pseudomonas putida
82200
-
-
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-cyclohexen-1-one + NADH
-
678118
Pseudomonas putida
? + NAD+
-
-
-
?
codeinone + NADH
-
678118
Pseudomonas putida
hydrocodone + NAD+
-
-
-
?
morphinone + NADH
-
678118
Pseudomonas putida
hydromorphone + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 41100
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
flavin
dependent, contains one molar equivalent of noncovalently bound FMN per enzyme subunit
Pseudomonas putida
NADH
dependent
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
flavin
dependent, contains one molar equivalent of noncovalently bound FMN per enzyme subunit
Pseudomonas putida
NADH
dependent
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cortisone
-
Pseudomonas putida
progesterone
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41100
-
2 * 41100
Pseudomonas putida
82200
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-cyclohexen-1-one + NADH
-
678118
Pseudomonas putida
? + NAD+
-
-
-
?
codeinone + NADH
-
678118
Pseudomonas putida
hydrocodone + NAD+
-
-
-
?
morphinone + NADH
-
678118
Pseudomonas putida
hydromorphone + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 41100
Pseudomonas putida
Other publictions for EC 1.3.1.42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745029
Xin
A putative 12-oxophytodienoat ...
Camellia sinensis
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18-24
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1
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5
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6
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1
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6
-
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-
-
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-
1
1
1
1
-
-
746104
Dong
Wheat oxophytodienoate reduct ...
Triticum aestivum
Plant Physiol.
161
1217-1228
2013
-
-
1
-
1
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2
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4
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1
1
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1
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2
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1
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2
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1
1
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-
1
1
3
3
1
-
-
724151
Abu-Romman
-
Molecular cloning and expressi ...
Hordeum vulgare
Aust. J. Crop Sci.
6
649-655
2012
-
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1
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1
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1
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1
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1
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1
-
-
1
-
-
726148
Li
Comparative characterization, ...
Oryza sativa
Plant Cell Rep.
30
981-995
2011
-
-
1
-
-
-
-
5
-
-
-
-
-
5
-
-
-
-
-
7
-
-
6
-
1
-
-
5
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
7
-
-
6
-
1
-
-
5
1
-
-
-
-
-
-
-
-
-
726461
Han
Crystal structure of Arabidops ...
Arabidopsis thaliana
Proteins
79
3236-3241
2011
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
-
-
-
-
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-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699588
Breithaupt
Structural basis of substrate ...
Solanum lycopersicum
J. Mol. Biol.
392
1266-1277
2009
-
-
1
1
3
-
1
-
-
-
-
4
-
3
-
-
-
-
-
-
-
-
4
1
-
-
-
-
1
-
-
3
-
-
-
-
-
1
5
2
3
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
700835
Beynon
The role of oxophytodienoate r ...
Arabidopsis thaliana
Plant Physiol.
151
253-261
2009
1
1
1
-
-
-
-
-
1
-
-
1
-
3
-
-
1
-
-
2
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
3
3
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
3
-
5
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711596
Li
Phylogenetic analysis, structu ...
Arabidopsis thaliana, Chlamydomonas reinhardtii, Medicago truncatula, Oryza sativa, Physcomitrella patens, Picea sitchensis, Populus trichocarpa, Selaginella moellendorffii, Sorghum bicolor, Volvox carteri, Zea mays
BMC Evol. Biol.
9
90
2009
-
-
11
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
22
22
-
-
-
689661
Gu
-
Overexpression of ZmOPR1 in Ar ...
Zea mays
Plant Sci.
174
124-130
2008
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689685
Tani
Identification of the OsOPR7 g ...
Oryza sativa
Planta
227
517-526
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690761
Yara
Resistance to Magnaporthe gris ...
Oryza sativa
Biochem. Biophys. Res. Commun.
376
460-465
2008
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684429
Hall
Asymmetric bioreduction of act ...
Solanum lycopersicum
Angew. Chem.
46
3934-3937
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
29
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689081
Engelberth
Insect elicitors and exposure ...
Zea mays
Mol. Plant Microbe Interact.
20
707-716
2007
1
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
8
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676813
Breithaupt
Crystal structure of 12-oxophy ...
Solanum lycopersicum
Proc. Natl. Acad. Sci. USA
103
14337-14342
2006
-
-
-
1
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
3
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
3
-
-
-
2
-
-
-
-
-
-
-
-
-
-
677040
Fox
X-ray structure of Arabidopsis ...
Arabidopsis thaliana
Proteins
61
206-208
2005
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
-
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1
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1
1
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-
-
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-
-
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-
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-
-
-
-
-
-
656802
Matsui
Structure and expression of 12 ...
Pisum sativum
Mol. Genet. Genomics
271
1-10
2004
1
-
1
-
-
-
-
-
-
-
-
2
-
8
-
-
1
-
-
3
-
-
8
-
1
-
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
3
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657324
Malone
X-ray structure of Arabidopsis ...
Arabidopsis thaliana
Proteins
58
243-245
2004
-
-
1
1
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657134
Sobajima
Cloning and characterization o ...
Oryza sativa
Planta
216
692-698
2003
-
-
1
-
-
-
-
-
-
-
1
2
-
1
-
-
1
-
-
1
-
-
3
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
1
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656976
Ishiga
Expression of the 12-oxophytod ...
Pisum sativum
Plant Cell Physiol.
43
1210-1220
2002
1
-
1
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
2
1
-
3
-
1
-
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
2
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656998
Strassner
Characterization and cDNA-micr ...
Arabidopsis thaliana, Solanum lycopersicum
Plant J.
32
585-601
2002
-
-
2
-
1
-
-
-
4
2
-
7
-
4
-
-
2
-
-
3
-
-
16
-
2
-
-
-
2
-
-
4
-
-
-
-
-
2
4
-
1
-
-
-
-
-
4
2
-
7
-
-
-
2
-
3
-
-
16
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
390780
Breithaupt
X-ray structure of 12-oxophyto ...
Solanum lycopersicum
Structure
9
419-429
2001
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390778
Costa
An Arabidopsis gene induced by ...
Arabidopsis sp., Arabidopsis thaliana, Capnoides sempervirens, Saccharomyces cerevisiae, Saccharomyces pastorianus, Zea mays
Plant Mol. Biol.
44
61-71
2000
-
-
3
1
-
-
-
2
-
-
-
4
-
8
-
-
1
-
-
5
-
-
15
1
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
2
-
-
-
4
-
-
-
1
-
5
-
-
15
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
390779
Schaller
12-Oxophytodienoate reductase ...
Arabidopsis thaliana, Capnoides sempervirens, Saccharomyces cerevisiae
Planta
210
979-984
2000
-
-
1
-
-
-
-
1
-
-
-
2
-
5
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
3
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390777
Biesgen
Structure and regulation of OP ...
Arabidopsis thaliana, Bryonia dioica
Planta
208
155-165
1999
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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