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Literature summary for 1.3.1.42 extracted from
- An Arabidopsis gene induced by wounding functionally homologous to flavoprotein oxidoreductases (2000), Plant Mol. Biol., 44, 61-71.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| c-DNA library, gene OPR3 identified | Arabidopsis sp. |
| cloning and expression of a gene encoding old yellow enzyme | Saccharomyces pastorianus |
| OPR1 and OPR2, two closely related genes encodes 12-oxophytodienoic acid-10,11-reductases | Arabidopsis thaliana |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
12-oxo-cis-phythodienoic acid | - |
Arabidopsis sp. |  |
| 2.5 | - |
cyclohexenone | - |
Arabidopsis sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 12-oxo-cis-phythodienoic acid + NADPH | Zea mays | - |
cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+ | - |
? | |
| 12-oxo-cis-phythodienoic acid + NADPH | Arabidopsis sp. | enzyme induced upon wounding | cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+ | OPC-8:0 | ? | |
| 12-oxo-cis-phythodienoic acid + NADPH | Arabidopsis thaliana | wound-induced gene activation | cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+ | OPC-8:0 | ? | |
| 9R,13R-12-oxo-phytodienoic acid + NADPH | Arabidopsis sp. | oxylipin | ? + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis sp. | - |
- |
- |
| Arabidopsis thaliana | - |
(L.) Heynta wild-type ecotype Columbia(Col-0) | - |
| Capnoides sempervirens | - |
rock harlequin | - |
| Saccharomyces cerevisiae | - |
yeast | - |
| Saccharomyces pastorianus | - |
- |
- |
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Arabidopsis sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis sp. | - |
| root | OPR1 and OPR2, OPR3 poorly expressed | Arabidopsis sp. | - |
| seedling | - |
Zea mays | - |
| seedling | - |
Arabidopsis sp. | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 12-oxo-cis-phythodienoic acid + NADPH | - |
Zea mays | cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+ | - |
? | |
| 12-oxo-cis-phythodienoic acid + NADPH | enzyme induced upon wounding | Arabidopsis sp. | cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+ | OPC-8:0 | ? | |
| 12-oxo-cis-phythodienoic acid + NADPH | wound-induced gene activation | Arabidopsis thaliana | cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+ | OPC-8:0 | ? | |
| 12-oxo-trans-phythodienoic acid + alpha-NADPH | - |
Arabidopsis sp. | trans-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+ | - |
? | |
| 12-oxo-trans-phythodienoic acid + beta-NADPH | - |
Arabidopsis sp. | trans-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+ | - |
? | |
| 15-deoxy-DELTA12,14-prostaglandin J2 + alpha-NADPH | PGJ2, an analogue of OPDA, rates of reaction with alpha-NADPH twenty times lower than those with beta-NADPH | Arabidopsis sp. | ? + alpha-NADPH | - |
? | |
| 15-deoxy-DELTA12,14-prostaglandin J2 + beta-NADPH | PGJ2, an analogue of OPDA | Arabidopsis sp. | ? + NADP+ | - |
? | |
| 9R,13R-12-oxo-phytodienoic acid + NADPH | oxylipin | Arabidopsis sp. | ? + NADP+ | - |
? | |
| beta-oestradiol + NADPH | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
| beta-oestradiol + NADPH | very low but significant oxidoreductase activity, detected in micromolar quantities | Arabidopsis sp. | ? + NADP+ | - |
? | |
| cyclohexenone + alpha-NADPH | - |
Arabidopsis sp. | ? + NADP+ | - |
? | |
| cyclohexenone + beta-NADPH | - |
Saccharomyces cerevisiae | ? | - |
? | |
| cyclohexenone + beta-NADPH | - |
Arabidopsis sp. | ? | - |
? | |
| additional information | cyclohexanone, testosterone and progesterone are no substrates | Arabidopsis sp. | ? | - |
? | |
| testosterone + NADPH + H+ | - |
Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | homodimeric protein with one molecule of flavin per monomer | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 12-oxophytodienoate-10,11-reductase | - |
Arabidopsis sp. |
| 12-oxophytodienoate-10,11-reductase | - |
Capnoides sempervirens |
| old yellow enzyme | - |
Saccharomyces pastorianus |
| old yellow enzyme | - |
Saccharomyces cerevisiae |
| OPR | - |
Arabidopsis sp. |
| OPR1 | - |
Arabidopsis thaliana |
| OPR1 | - |
Arabidopsis sp. |
| OPR1 | - |
Capnoides sempervirens |
| OPR2 | - |
Arabidopsis thaliana |
| OPR2 | - |
Arabidopsis sp. |
| OPR3 oxidoreductase | - |
Arabidopsis sp. |
| OYE | - |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | - |
Arabidopsis sp. |
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