Inhibitors | Comment | Organism | Structure |
---|---|---|---|
monoiodoacetate | - |
Pseudomonas cruciviae | |
p-chloromercuribenzoate | - |
Pseudomonas cruciviae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzyme III, gel filtration | Pseudomonas cruciviae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas cruciviae | - |
S93B1, 2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzymes I, II, III | - |
Purification (Comment) | Organism |
---|---|
2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzyme III | Pseudomonas cruciviae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzyme III | Pseudomonas cruciviae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-6-oxo-phenylhexa-2,4-dienoate + NADPH | - |
Pseudomonas cruciviae | 2,6-dioxo-6-phenylhexanoate + NADP+ | - |
? | |
2-methoxy-6-oxo-6-phenylhexa-2,3-dienoic acid methyl ester + NADPH | enzymes I and II | Pseudomonas cruciviae | 2-methoxy-6-oxo-6-phenylhexa-2-enoic acid methyl ester + NADP+ | - |
? | |
2-methoxy-6-oxo-6-phenylhexa-2,4-dienoic acid methyl ester + NADPH | enzyme III | Pseudomonas cruciviae | 2-methoxy-6-oxo-6-phenylhexa-2-enoic acid methyl ester + NADP+ | - |
? | |
ring fission product of 3-methoxycatechol + NADPH | enzymes I and II | Pseudomonas cruciviae | ? | - |
? | |
ring fission products from 3-isopropylcatechol + NADPH | enzymes I, II and III | Pseudomonas cruciviae | ? | - |
? | |
ring fission products from 3-methylcatechol + NADPH | enzymes I, II and III | Pseudomonas cruciviae | ? | - |
? | |
ring fission products from biphenyl-2,3-diol + NADPH | enzymes I, II and III | Pseudomonas cruciviae | ? | - |
? | |
ring fission products of catechol + NADPH | enzymes I and II | Pseudomonas cruciviae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas cruciviae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzyme III | Pseudomonas cruciviae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pseudomonas cruciviae |