Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
F149A | the mutant is not able to optimally position the cofactor for hydride transfer, which correlates with the 30fold decrease in kcat | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00433 | - |
2-dodecenoyl-CoA | mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) | Mycobacterium tuberculosis | |
0.0054 | - |
2-dodecenoyl-CoA | wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) | Mycobacterium tuberculosis | |
0.0152 | - |
NADH | mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) | Mycobacterium tuberculosis | |
0.0352 | - |
NADH | wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dodecenoyl-acyl carrier protein + NADH | - |
Mycobacterium tuberculosis | dodecanoyl-acyl carrier protein + NAD+ | - |
? | |
2-trans,4-trans-decadienoyl-CoA + NADPH + H+ | - |
Mycobacterium tuberculosis | (4E)-dec-4-enoyl-CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enoyl reductase | - |
Mycobacterium tuberculosis |
InhA | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
(4E)-dec-4-enoyl-CoA | mutant enzyme F149A, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) | Mycobacterium tuberculosis | |
3.17 | - |
(4E)-dec-4-enoyl-CoA | wild type enzyme, at 25°C in 30 mM PIPES and 150 mM NaCl (pH 6.8) | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycobacterium tuberculosis |