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Literature summary for 1.3.1.38 extracted from

  • Das, A.K.; Uhler, M.D.; Hajra, A.K.
    Molecular cloning and expression of mammalian peroxisomal trans-2-enoyl-coenzyme A reductase cDNAs (2000), J. Biol. Chem., 275, 24333-24340.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens
-
Mus musculus
expression as His-tagged protein in Escherichia coli, sequence analysis Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide native and recombinant enzyme Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
trans-2-decenoyl-CoA
-
Cavia porcellus
0.018
-
trans-2-decenoyl-CoA recombinant enzyme Cavia porcellus
0.038
-
NADPH
-
Cavia porcellus
0.053
-
NADPH recombinant enzyme Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Cavia porcellus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32300
-
? * 32300, sequence determination Homo sapiens
32600
-
? * 32600, sequence determination Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cavia porcellus key enzyme in fatty acid chain elongation pathway ?
-
?

Organism

Organism UniProt Comment Textmining
Cavia porcellus Q9JIF5 guinea pig
-
Homo sapiens Q9BY49
-
-
Mus musculus Q99MZ7
-
-

Purification (Commentary)

Purification (Comment) Organism
associated with peroxisomal acyldihydroxyacetone phosphate reductase Cavia porcellus
partial Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Cavia porcellus
-
liver
-
Homo sapiens
-
liver
-
Cavia porcellus
-
liver
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0752
-
partially purified Cavia porcellus
0.346
-
purified recombinant enzyme Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information key enzyme in fatty acid chain elongation pathway Cavia porcellus ?
-
?
trans-2-decenoyl-CoA + NADPH highest activity with, native and recombinant enzyme Cavia porcellus decanoyl-CoA + NADP+
-
?
trans-2-hexenoyl-CoA + NADPH 50% activity compared to that with trans-2-decenoyl-CoA, native and recombinant enzyme Cavia porcellus hexanoyl-CoA + NADP+
-
?
trans-2-octenoyl-CoA + NADPH 50% activity compared to that with trans-2-decenoyl-CoA, native and recombinant enzyme Cavia porcellus octanoyl-CoA + NADP+
-
?

Subunits

Subunits Comment Organism
? ? * 32500-40000, sequence determination and SDS-PAGE Cavia porcellus
? ? * 32600, sequence determination Mus musculus
? ? * 32300, sequence determination Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Cavia porcellus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens
NADPH
-
Mus musculus
NADPH specific for Cavia porcellus