Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Mus musculus |
expression as His-tagged protein in Escherichia coli, sequence analysis | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | native and recombinant enzyme | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
trans-2-decenoyl-CoA | - |
Cavia porcellus | |
0.018 | - |
trans-2-decenoyl-CoA | recombinant enzyme | Cavia porcellus | |
0.038 | - |
NADPH | - |
Cavia porcellus | |
0.053 | - |
NADPH | recombinant enzyme | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Cavia porcellus | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32300 | - |
? * 32300, sequence determination | Homo sapiens |
32600 | - |
? * 32600, sequence determination | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cavia porcellus | key enzyme in fatty acid chain elongation pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | Q9JIF5 | guinea pig | - |
Homo sapiens | Q9BY49 | - |
- |
Mus musculus | Q99MZ7 | - |
- |
Purification (Comment) | Organism |
---|---|
associated with peroxisomal acyldihydroxyacetone phosphate reductase | Cavia porcellus |
partial | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Cavia porcellus | - |
liver | - |
Homo sapiens | - |
liver | - |
Cavia porcellus | - |
liver | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0752 | - |
partially purified | Cavia porcellus |
0.346 | - |
purified recombinant enzyme | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | key enzyme in fatty acid chain elongation pathway | Cavia porcellus | ? | - |
? | |
trans-2-decenoyl-CoA + NADPH | highest activity with, native and recombinant enzyme | Cavia porcellus | decanoyl-CoA + NADP+ | - |
? | |
trans-2-hexenoyl-CoA + NADPH | 50% activity compared to that with trans-2-decenoyl-CoA, native and recombinant enzyme | Cavia porcellus | hexanoyl-CoA + NADP+ | - |
? | |
trans-2-octenoyl-CoA + NADPH | 50% activity compared to that with trans-2-decenoyl-CoA, native and recombinant enzyme | Cavia porcellus | octanoyl-CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | ? * 32500-40000, sequence determination and SDS-PAGE | Cavia porcellus |
? | ? * 32600, sequence determination | Mus musculus |
? | ? * 32300, sequence determination | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens | |
NADPH | - |
Mus musculus | |
NADPH | specific for | Cavia porcellus |