Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Escherichia coli | |
iodoacetic acid | - |
Escherichia coli | |
additional information | Zn2+, Fe3+, Mn2+, Mg2+, Ca2+, NH4+, Na+ | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-2-hexenoyl-CoA + NADPH + H+ | Escherichia coli | - |
hexanoyl-CoA + NADP+ | - |
? | |
trans-2-octenoyl-CoA + NADPH + H+ | Escherichia coli | - |
octanoyl-CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH + H+ | inducible by growth on oleic acid | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.49 | - |
partially purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, pH 7.0 | Escherichia coli |
0°C, pH 7.0, 1 mg per ml, 70% of activity remaining after 44 h | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no reduction of trans-2-tetradecenoyl-CoA | Escherichia coli | ? | - |
? | |
trans-2-hexenoyl-CoA + NADPH | - |
Escherichia coli | hexanoyl-CoA + NADP+ | - |
? | |
trans-2-hexenoyl-CoA + NADPH + H+ | - |
Escherichia coli | hexanoyl-CoA + NADP+ | - |
? | |
trans-2-octenoyl-CoA + NADPH | - |
Escherichia coli | octanoyl-CoA + NADP+ | - |
? | |
trans-2-octenoyl-CoA + NADPH + H+ | - |
Escherichia coli | octanoyl-CoA + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 min, pH 6.0-8.0 | Escherichia coli |
56 | - |
10 min, pH 6.0, 50% inactivation but reactivation at pH 8.0 | Escherichia coli |
60 | - |
10 min, inactivation | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for | Escherichia coli |