Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no flavoprotein | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | i.e. DTNB, inhibition can be prevented by preincubation with NADPH, thus a SH-group is involved in cofactor binding | Escherichia coli | |
bovine serum albumin | - |
Escherichia coli | |
decanoyl-CoA | - |
Escherichia coli | |
lauroyl-CoA | - |
Escherichia coli | |
additional information | not inhibitory: methylglyoxal | Escherichia coli | |
myristoyl-CoA | - |
Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
NADP+ | - |
Escherichia coli | |
octanoyl-CoA | - |
Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli | |
palmitoleoyl-CoA | - |
Escherichia coli | |
palmitoyl-CoA | - |
Escherichia coli | |
Saturated CoA analogs | - |
Escherichia coli | |
Triton X-100 | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
trans-2-dodecenoyl-CoA | - |
Escherichia coli | |
0.0049 | - |
NADPH | - |
Escherichia coli | |
0.0071 | - |
trans-2-decenoyl-CoA | - |
Escherichia coli | |
0.011 | - |
trans-2-octenoyl-CoA | - |
Escherichia coli | |
0.048 | - |
trans-2-hexenoyl-CoA | - |
Escherichia coli | |
0.072 | - |
trans-2-crotonyl-CoA | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Escherichia coli |
80000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-2-hexenoyl-CoA + NADPH + H+ | Escherichia coli | - |
hexanoyl-CoA + NADP+ | - |
? | |
trans-2-octenoyl-CoA + NADPH + H+ | Escherichia coli | - |
octanoyl-CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, pH 8.0, 10% v/v glycerol | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADPH | - |
Escherichia coli | butanoyl-CoA + NADP+ | - |
? | |
additional information | activity decreases with increasing chain length of substrates | Escherichia coli | ? | - |
? | |
trans-2-decenoyl-CoA + NADPH | - |
Escherichia coli | decanoyl-CoA + NADP+ | - |
? | |
trans-2-dodecenoyl-CoA | - |
Escherichia coli | ? | - |
r | |
trans-2-hexenoyl-CoA + NADPH | - |
Escherichia coli | hexanoyl-CoA + NADP+ | - |
? | |
trans-2-hexenoyl-CoA + NADPH + H+ | - |
Escherichia coli | hexanoyl-CoA + NADP+ | - |
? | |
trans-2-octenoyl-CoA + NADPH | - |
Escherichia coli | octanoyl-CoA + NADP+ | - |
? | |
trans-2-octenoyl-CoA + NADPH + H+ | - |
Escherichia coli | octanoyl-CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00054 | - |
palmitoleoyl-CoA | - |
Escherichia coli | |
0.0025 | - |
palmitoyl-CoA | - |
Escherichia coli | |
0.0092 | - |
myristoyl-CoA | - |
Escherichia coli | |
0.045 | - |
lauroyl-CoA | - |
Escherichia coli | |
0.063 | - |
decanoyl-CoA | - |
Escherichia coli | |
0.198 | - |
octanoyl-CoA | - |
Escherichia coli |