Literature summary for 1.3.1.33 extracted from

Masuda, S.; Ikeda, R.; Masuda, T.; Hashimoto, H.; Tsuchiya, T.; Kojima, H.; Nomata, J.; Fujita, Y.; Mimuro, M.; Ohta, H.; Takamiya, K.
Prolamellar bodies formed by cyanobacterial protochlorophyllide oxidoreductase in Arabidopsis (2009), Plant J., 58, 952-960.

Search for more literature for 1.3.1.33

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli cells	Synechocystis sp. PCC 6803
expression in Escherichia coli cells	Gloeobacter violaceus
yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells	Arabidopsis thaliana
yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells, for the construction of transgenic plants pDONR/ZeO and pGWB2 vectors are used	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00015	-	protochlorophyllide	-	Arabidopsis thaliana
0.00039	-	protochlorophyllide	-	Arabidopsis thaliana
0.0005	-	protochlorophyllide	-	Gloeobacter violaceus
0.0075	-	NADPH	-	Synechocystis sp. PCC 6803
0.01083	-	protochlorophyllide	-	Synechocystis sp. PCC 6803
0.0196	-	NADPH	-	Gloeobacter violaceus
0.0268	-	NADPH	-	Arabidopsis thaliana
0.0595	-	NADPH	-	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-
etioplast prolamellar body	-	Arabidopsis thaliana	9541	-
plastid	-	Arabidopsis thaliana	9536	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protochlorophyllide + NADPH + H+	Synechocystis sp. PCC 6803	-	chlorophyllide + NADP+	-	?
protochlorophyllide + NADPH + H+	Gloeobacter violaceus	-	chlorophyllide + NADP+	-	?
protochlorophyllide + NADPH + H+	Arabidopsis thaliana	-	chlorophyllide + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	O48741	-	-
Arabidopsis thaliana	P21218	-	-
Arabidopsis thaliana	Q42536	-	-
Gloeobacter violaceus	-	-	-
Synechocystis sp. PCC 6803	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a chitin bead column, the chitin-intein tag is removed	Arabidopsis thaliana
using His-Bind resin	Synechocystis sp. PCC 6803
using His-Bind resin	Gloeobacter violaceus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
protochlorophyllide + NADPH + H+	-	Synechocystis sp. PCC 6803	chlorophyllide + NADP+	-	?
protochlorophyllide + NADPH + H+	-	Gloeobacter violaceus	chlorophyllide + NADP+	-	?
protochlorophyllide + NADPH + H+	-	Arabidopsis thaliana	chlorophyllide + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
POR	-	Synechocystis sp. PCC 6803
POR	-	Gloeobacter violaceus
POR	-	Arabidopsis thaliana
PORA	-	Arabidopsis thaliana
PORB	-	Arabidopsis thaliana
PORC	-	Arabidopsis thaliana
protochlorophyllide oxidoreductase	-	Synechocystis sp. PCC 6803
protochlorophyllide oxidoreductase	-	Gloeobacter violaceus
protochlorophyllide oxidoreductase	-	Arabidopsis thaliana
protochlorophyllide oxidoreductase A	-	Arabidopsis thaliana
protochlorophyllide oxidoreductase B	-	Arabidopsis thaliana
protochlorophyllide oxidoreductase C	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
23	-	activity assay at room temperature	Synechocystis sp. PCC 6803
23	-	activity assay at room temperature	Gloeobacter violaceus
23	-	activity assay at room temperature	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	activity assay	Synechocystis sp. PCC 6803
7.5	-	activity assay	Gloeobacter violaceus
7.5	-	activity assay	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Synechocystis sp. PCC 6803
NADPH	-	Gloeobacter violaceus
NADPH	-	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)