Any feedback?
Literature summary for 1.3.1.33 extracted from
- Overexpression and characterization of dark-operative protochlorophyllide reductase from Rhodobacter capsulatus (2005), Biochim. Biophys. Acta, 1708, 229-237.
General Stability
| General Stability | Organism |
|---|---|
| rapid loss of activity during attempts to purify | Rhodobacter capsulatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | stable enzyme assay system by mixing recombinant enzyme components L-protein and NB-protein under anaerobic conditiions | Rhodobacter capsulatus | |
| 0.0106 | - |
protochlorophyllide | pH 7.4, 34°C | Rhodobacter capsulatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
| 36046 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
| 48671 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
| 52000 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
| 57191 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
| 60000 | - |
x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodobacter capsulatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both activities of the components protein L and proteins NB are rapidly lost during purification procedures, such as affinity chromatography with S-protein agarose. Attempts to purify the active components have so far been unsuccessful | Rhodobacter capsulatus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| enzyme in crude extracts stable for more than 6 months when maintained anaerobically at 4°C, with no significant loss of activity | Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protochlorophyllide + NADPH | - |
Rhodobacter capsulatus | chlorophyllide + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE, x * 36046, calculated, S-tagged L-protein, x * 52000, SDS-PAGE, x * 48671, calculated, S-tagged N-protein, x * 60000, SDS-PAGE, x * 57191, calculated, B-protein | Rhodobacter capsulatus |
| More | recombinant enzyme component L-protein forms a dimer, recombinant components NB-protein form a heterotetramer, gel filtration | Rhodobacter capsulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DPOR | - |
Rhodobacter capsulatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | - |
Rhodobacter capsulatus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
