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Literature summary for 1.3.1.33 extracted from

  • Lebedev, N.; Karginova, O.; McIvor, W.; Timko, M.P.
    Tyr275 and Lys279 Stabilize NADPH within the Catalytic Site of NADPH:Protochlorophyllide Oxidoreductase and Are Involved in the Formation of the Enzyme Photoactive State (2001), Biochemistry, 40, 12562-12574.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the nuclear gene Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00018
-
protochlorophyllide
-
Pisum sativum
0.011
-
NADPH
-
Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protochlorophyllide + NADPH Pisum sativum
-
chlorophyllide + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
pea
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant pea enzyme, heterologously expressed in Escherichia coli Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
C8-ethyl-C13(2)-(r)-protochlorophyllide + NADPH stereoisomer of the substrate Pisum sativum ? + NADP+
-
?
protochlorophyllide + NADPH
-
Pisum sativum chlorophyllide + NADP+
-
?

Synonyms

Synonyms Comment Organism
POR
-
Pisum sativum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Pisum sativum