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Literature summary for 1.3.1.33 extracted from

  • Griffiths, W.T.
    Substrate-specificity studies on protochlorophyllide reductase in barley (Hordeum vulgare) etioplast membranes (1980), Biochem. J., 186, 267-278.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
etioplast
-
Hordeum vulgare 9513
-
etioplast prolamellar body
-
Hordeum vulgare 9541
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity Hordeum vulgare
Zn2+ removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate, activity can be restored by replacing the magnesium with zinc, whereas nickel, copper or cobalt fail to restore substrate activity Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Hordeum vulgare naturally occurring esterified protochlorophyllide and chemically prepared protochlorophyllide methyl ester are not substrates for the enzyme, removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate ?
-
?
protochlorophyllide + NADPH Hordeum vulgare biosynthetic pathway leading to chlorophyll a chlorophyllide + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
barley
-
Hordeum vulgare
-
L., cultivar Proctor
-

Purification (Commentary)

Purification (Comment) Organism
-
Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information naturally occurring esterified protochlorophyllide and chemically prepared protochlorophyllide methyl ester are not substrates for the enzyme, removal of Mg2+ from the protochlorophyllide leads to inactivity of the compound as a substrate Hordeum vulgare ?
-
?
protochlorophyllide + NADPH
-
Hordeum vulgare chlorophyllide + NADP+
-
?
protochlorophyllide + NADPH biosynthetic pathway leading to chlorophyll a Hordeum vulgare chlorophyllide + NADP+
-
?

Cofactor

Cofactor Comment Organism Structure
NADPH no reaction with NADH Hordeum vulgare