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Literature summary for 1.3.1.32 extracted from

  • Gaal, A.B.; Neujahr, H.Y.
    Maleylacetate reductase from Trichosporon cutaneum (1980), Biochem. J., 185, 783-786.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 total inhibition, reversed by thiol Cutaneotrichosporon cutaneum
maleylacetate above 300 mM Cutaneotrichosporon cutaneum
p-hydroxymercuribenzoate total inhibition, reversed by thiol Cutaneotrichosporon cutaneum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Cutaneotrichosporon cutaneum
81000
-
gel filtration Cutaneotrichosporon cutaneum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-maleylacetate + NADPH Pseudomonas putida
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Cutaneotrichosporon cutaneum
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Sporotrichum pulverulentum
-
3-oxoadipate + NADP+
-
ir

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
phenol-utilizing yeast
-
Pseudomonas putida
-
-
-
Sporotrichum pulverulentum
-
lignin-degrading white-rot fungus
-

Purification (Commentary)

Purification (Comment) Organism
-
Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34.2
-
-
Cutaneotrichosporon cutaneum

Storage Stability

Storage Stability Organism
-25°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several months without loss of activity Cutaneotrichosporon cutaneum
5°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several days without loss of activity Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-maleylacetate + NAD(P)H
-
Pseudomonas putida 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Cutaneotrichosporon cutaneum 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Sporotrichum pulverulentum 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NADPH
-
Pseudomonas putida 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Cutaneotrichosporon cutaneum 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Sporotrichum pulverulentum 3-oxoadipate + NADP+
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Cutaneotrichosporon cutaneum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.8
-
Cutaneotrichosporon cutaneum

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas putida
NADH
-
Cutaneotrichosporon cutaneum
NADPH
-
Cutaneotrichosporon cutaneum
NADPH
-
Sporotrichum pulverulentum