Literature summary for 1.3.1.31 extracted from

Classen, T.; Pietruszka, J.; Schuback, S.
Revisiting the enantioselective sequence patterns in enoate reductases (2013), ChemCatChem, 5, 711-713.

No PubMed abstract available

Search for more literature for 1.3.1.31

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Photorhabdus luminescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40400	-	-	Photorhabdus luminescens
42500	-	x * 42500, SDS-PAGE	Photorhabdus luminescens

Organism

Organism	UniProt	Comment	Textmining
Photorhabdus luminescens	-	subspecies Photorhabdus luminescens laumondii	-
Solanum lycopersicum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Photorhabdus luminescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(1-nitroprop-1-en-2-yl)benzene + NADP+	-	Photorhabdus luminescens	?	-	?
(1-nitroprop-1-en-2-yl)benzene + NADP+	-	Solanum lycopersicum	[(2R)-1-nitropropan-2-yl]benzene + NADPH + H+	product of isoform OPR1	?
(1-nitroprop-1-en-2-yl)benzene + NADP+	-	Photorhabdus luminescens	[(2S)-1-nitropropan-2-yl]benzene + NADPH + H+	-	?
(1-nitroprop-1-en-2-yl)benzene + NADP+	-	Solanum lycopersicum	[(2S)-1-nitropropan-2-yl]benzene + NADPH + H+	product of isoform OPR3	?
(E)-(2-nitroprop-1-en-1-yl)benzene + NADP+	-	Photorhabdus luminescens	?	-	?
(E)-(2-nitroprop-1-en-1-yl)benzene + NADP+	-	Photorhabdus luminescens	[(3S)-3-nitrobutyl]benzene + NADPH + H+	-	?
(E)-ethyl 2-methyl-4-oxopent-2-enoate + NADP+	-	Photorhabdus luminescens	?	-	?
(E)-ethyl 2-methyl-4-oxopent-2-enoate + NADP+	-	Photorhabdus luminescens	ethyl (2R)-2-methyl-4-oxopentanoate + NADPH + H+	-	?
methyl 2-phenylacrylate + NADP+	-	Photorhabdus luminescens	methyl (2R)-2-methyl-3-phenylpropanoate + NADPH + H+	-	?
methyl 2-phenylacrylate + NADP+	-	Photorhabdus luminescens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 42500, SDS-PAGE	Photorhabdus luminescens
?	x * 40400, calculated from amino acid sequence	Photorhabdus luminescens

Synonyms

Synonyms	Comment	Organism
NCR	-	Photorhabdus luminescens
OPR1	-	Solanum lycopersicum
OPR3	-	Solanum lycopersicum

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Photorhabdus luminescens
NADP+	-	Solanum lycopersicum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)