Literature summary for 1.3.1.31 extracted from

Kuno, S.; Bacher, A.; Simon, H.
Structure of enoate reductase from a Clostridium tyrobutyricum (C. spec. La1) (1985), Biol. Chem. Hoppe-Seyler, 366, 463-472.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	iron-sulfur protein, contains 4.7 mol Fe per mol of subunit	Clostridium tyrobutyricum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	12 * 70000, SDS-PAGE	Clostridium tyrobutyricum
73000	-	12 * 73000, equilibrium sedimentation in guanidine hydrochloride	Clostridium tyrobutyricum
800000	-	gel filtration	Clostridium tyrobutyricum
940000	-	equilibrium ultracentrifugation	Clostridium tyrobutyricum

Organism

Organism	UniProt	Comment	Textmining
Clostridium tyrobutyricum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Clostridium tyrobutyricum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10.6	-	-	Clostridium tyrobutyricum

Storage Stability

Storage Stability	Organism
-18°C, pH 7, buffer containing 10 mM phosphate and 0.1 M KCl, formation of a dissociation product sedimenting at about 10 S	Clostridium tyrobutyricum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
butenoate + NADH	-	Clostridium tyrobutyricum	butanoate + NAD+	-	?

Subunits

Subunits	Comment	Organism
dodecamer	12 * 70000, SDS-PAGE	Clostridium tyrobutyricum
dodecamer	12 * 73000, equilibrium sedimentation in guanidine hydrochloride	Clostridium tyrobutyricum

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Clostridium tyrobutyricum

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Release 2023.1 (January 2023)