Literature summary for 1.3.1.3 extracted from

Zhang, Y.; Li, X.; Xu, D.; Wu, M.; Dai, Y.; Xia, M.; Wang, D.
Cloning and characterization of steroid 5beta-reductase from the venom gland of Bufo bufo gargarizans (2021), Int. J. Biol. Macromol., 175, 67-78 .

Search for more literature for 1.3.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bufo gargarizans

Protein Variants

Protein Variants	Comment	Organism
Y132G	10% increase in catalytic efficiency	Bufo gargarizans
Y132G/V309K	loss of activity	Bufo gargarizans
Y132G/V309W	loss of activity	Bufo gargarizans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0063	-	progesterone	wild-type, pH 7.4, 37°C	Bufo gargarizans
0.0105	-	progesterone	mutant Y132G, pH 7.4, 37°C	Bufo gargarizans

Organism

Organism	UniProt	Comment	Textmining
Bufo gargarizans	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
parotid gland	-	Bufo gargarizans	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
progesterone + NADPH + H+	no substrate inhibition observed	Bufo gargarizans	5beta-pregnane-3,20-dione + NADP+	-	?

Subunits

Subunits	Comment	Organism
?	x * 37260, calculated from sequence, x * 90200, recombinant pCold-TF fusion tag tagged protein	Bufo gargarizans

Synonyms

Synonyms	Comment	Organism
SRD5beta	-	Bufo gargarizans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00197	-	progesterone	wild-type, pH 7.4, 37°C	Bufo gargarizans
0.00348	-	progesterone	mutant Y132G, pH 7.4, 37°C	Bufo gargarizans

pI Value

Organism	Comment	pI Value Maximum	pI Value
Bufo gargarizans	calculated from sequence	-	6.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.313	-	progesterone	wild-type, pH 7.4, 37°C	Bufo gargarizans
0.35	-	progesterone	mutant Y132G, pH 7.4, 37°C	Bufo gargarizans

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Release 2023.1 (January 2023)