Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain M15 | Digitalis lanata |
Crystallization (Comment) | Organism |
---|---|
5beta-POR, in the presence and absence of the cofactor NADP+, hanging drop vapour diffusion method, using 15% polyethylene glycol 4000, 0.1 M ammonium acetate, and 0.1 M sodium citrate, pH 5.6 | Digitalis lanata |
Protein Variants | Comment | Organism |
---|---|---|
Y179A | inactive | Digitalis lanata |
Y179F | inactive | Digitalis lanata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
progesterone | purified wild type enzyme | Digitalis lanata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Digitalis lanata | Q6PQJ9 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-Sepharose affinity column chromatography and Superdex 75 gel filtration | Digitalis lanata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.89 | - |
purified wild type enzyme, using progesterone as substrate | Digitalis lanata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + NADPH + H+ | - |
Digitalis lanata | 5beta-pregnane-3,20-dione + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5beta-POR | - |
Digitalis lanata |
progesterone 5beta-reductase | - |
Digitalis lanata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Digitalis lanata |