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Literature summary for 1.3.1.3 extracted from

  • Tomkins, G.M.
    The enzymatic reduction of DELTA4-3-ketosteroids (1957), J. Biol. Chem., 225, 13-24.
    View publication on PubMed

General Stability

General Stability Organism
bovine serum albumin stabilizes Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
p-Chloromercuribenzyl sulfonate 2 mM, complete inhibition, 50% inhibition in the presence of 10 mM glutathione Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
NADPH
-
Rattus norvegicus
0.034
-
cortisone
-
Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Cavia porcellus
-
-
-
Columba sp.
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
105 units/mg, 1 unit is defined as a change in optical density of 0.001 in 20 min Rattus norvegicus

Storage Stability

Storage Stability Organism
-10°C, stable for 2-3 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cortisone + NADPH + H+
-
Cavia porcellus 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
?
cortisone + NADPH + H+
-
Sus scrofa 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
?
cortisone + NADPH + H+
-
Bos taurus 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
?
cortisone + NADPH + H+
-
Oryctolagus cuniculus 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
?
cortisone + NADPH + H+
-
Columba sp. 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
?
cortisone + NADPH + H+ high substrate specificity Rattus norvegicus 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+
-
ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3
-
complete loss of activity after 24 h Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8 about 50% of activity maximum at pH 5.2 and 8 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH absolutely required Cavia porcellus
NADPH absolutely required Rattus norvegicus