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Literature summary for 1.3.1.3 extracted from

  • Van Doorn, E.J.; Nduaguba, J.C.; Clark, A.F.
    Studies on partially purified pig liver steroid DELTA4-5beta-reductase activity (1973), Can. J. Biochem., 51, 1661-1668.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5beta-dihydrotestosterone product inhibition Sus scrofa
iodoacetate
-
Sus scrofa
N-ethylmaleimide
-
Sus scrofa
NADP+ product inhibition Sus scrofa
p-chloromercuribenzenesulfonate
-
Sus scrofa
p-chloromercuribenzoate
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.085
-
NADPH
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sus scrofa 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000 30000 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Storage Stability

Storage Stability Organism
4°C, 72 h, 0.05 M Tris-maleate buffer, pH 7.4-8.4, stable Sus scrofa
4°C, pH 8, 32 days, 25% loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aldosterone + NADPH
-
Sus scrofa ?
-
?
additional information enzyme reduces a variety of C19 and C21 steroids, highest activity with aldosterone Sus scrofa ?
-
?
testosterone + NADPH + H+
-
Sus scrofa (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
Tris-maleate buffer Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5.7 8 pH 5.7: about 50% of activity maximum, pH 8.0: about 70% of activity maximum Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
4°C, 30 days, 25% loss of activity Sus scrofa