Literature summary for 1.3.1.29 extracted from

Jouanneau, Y.; Meyer, C.
Purification and characterization of an arene cis-dihydrodiol dehydrogenase endowed with broad substrate specificity toward polycyclic aromatic hydrocarbon dihydrodiols (2006), Appl. Environ. Microbiol., 72, 4726-4734.

Search for more literature for 1.3.1.29

Cloned(Commentary)

Cloned (Comment)	Organism
into pET15b and overexpressed as His-tagged protein in Escherichia coli BL21(DE3)	Novosphingobium aromaticivorans

Inhibitors

Inhibitors	Comment	Organism	Structure
3,4-Dihydroxyphenanthrene	-	Novosphingobium aromaticivorans
NADH	-	Novosphingobium aromaticivorans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00141	-	naphthalene 1,2-dihydrodiol	-	Novosphingobium aromaticivorans
0.00185	-	phenanthrene 3,4-dihydrodiol	-	Novosphingobium aromaticivorans
0.002	-	anthracene 1,2-dihydrodiol	-	Novosphingobium aromaticivorans
0.0022	-	chrysene 3,4-dihydrodiol	at pH 7.0	Novosphingobium aromaticivorans
0.0035	-	biphenyl 2,3-dihydrodiol	at pH 7.0	Novosphingobium aromaticivorans
0.0036	-	biphenyl 2,3-dihydrodiol	at pH 9.5	Novosphingobium aromaticivorans
0.0057	-	chrysene 3,4-dihydrodiol	at pH 9.5	Novosphingobium aromaticivorans
0.0071	-	benzo(a)anthracene 1,2-dihydrodiol	-	Novosphingobium aromaticivorans
0.16	-	NAD+	at pH 7.0, with biphenyl 2,3-dihydrodiol as substrate	Novosphingobium aromaticivorans
0.168	-	NAD+	at pH 7.0, with phenanthrene 3,4-dihydrodiol	Novosphingobium aromaticivorans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27731	-	27731, predicted from sequence analysis, 30000, SDS-PAGE	Novosphingobium aromaticivorans
103000	-	gel filtration	Novosphingobium aromaticivorans
110000	-	-	Novosphingobium aromaticivorans

Organism

Organism	UniProt	Comment	Textmining
Novosphingobium aromaticivorans	-	-	-
Novosphingobium aromaticivorans CHY-1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by Co2+-loaded immobilized metal affinity chromatography	Novosphingobium aromaticivorans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3-dihydroxy-2,3-dihydrobiphenyl + NAD+	-	Novosphingobium aromaticivorans	2,3-dihydroxybiphenyl + NADH + H+	-	?
2,3-dihydroxy-2,3-dihydrobiphenyl + NAD+	-	Novosphingobium aromaticivorans CHY-1	2,3-dihydroxybiphenyl + NADH + H+	-	?
anthracene 1,2-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans	anthracene 1,2-diol + NADH + H+	-	?
anthracene 1,2-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans CHY-1	anthracene 1,2-diol + NADH + H+	-	?
benzo(a)anthracene 1,2-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans	benzo(a)anthracene 1,2-diol + NADH + H+	-	?
benzo(a)anthracene 1,2-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans CHY-1	benzo(a)anthracene 1,2-diol + NADH + H+	-	?
benzo(a)pyrene-9,10-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans	9,10-dihydroxy-benzo(a)pyrene + NADH + H+	-	?
benzo(a)pyrene-9,10-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans CHY-1	9,10-dihydroxy-benzo(a)pyrene + NADH + H+	-	?
chrysene 3,4-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans	chrysene 3,4-diol + NADH + H+	-	?
chrysene 3,4-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans CHY-1	chrysene 3,4-diol + NADH + H+	-	?
fluoranthene 2,3-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans	2,3-dihydroxyfluoranthene + NADH + H+	-	?
naphthalene-1,2-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans	naphthalene-1,2-diol + NADH + H+	-	?
phenanthrene 3,4-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans	phenanthrene 3,4-diol + NADH + H+	-	?
pyrene 4,5-dihydrodiol + NAD+	-	Novosphingobium aromaticivorans	4,5-dihydroxypyrene + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
tetramer	27731, predicted from sequence analysis, 30000, SDS-PAGE	Novosphingobium aromaticivorans

Synonyms

Synonyms	Comment	Organism
arene cis-dihydrodiol dehydrogenase	-	Novosphingobium aromaticivorans
PDDH	-	Novosphingobium aromaticivorans
polycyclic aromatic hydrocarbons dihydrodiol dehydrogenase	-	Novosphingobium aromaticivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.25	-	fluoranthene 2,3-dihydrodiol	-	Novosphingobium aromaticivorans
6.35	-	pyrene 4,5-dihydrodiol	-	Novosphingobium aromaticivorans
7.9	-	benzo(a)pyrene-9,10-dihydrodiol	-	Novosphingobium aromaticivorans
9.1	-	benzo(a)anthracene 1,2-dihydrodiol	-	Novosphingobium aromaticivorans
16.7	-	phenanthrene 3,4-dihydrodiol	-	Novosphingobium aromaticivorans
20.3	-	chrysene 3,4-dihydrodiol	at pH 7.0	Novosphingobium aromaticivorans
21.6	-	anthracene 1,2-dihydrodiol	-	Novosphingobium aromaticivorans
24.6	-	biphenyl 2,3-dihydrodiol	at pH 7.0	Novosphingobium aromaticivorans
28.2	-	naphthalene 1,2-dihydrodiol	-	Novosphingobium aromaticivorans
218	-	chrysene 3,4-dihydrodiol	at pH 9.5	Novosphingobium aromaticivorans
321	-	biphenyl 2,3-dihydrodiol	at pH 9.5	Novosphingobium aromaticivorans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.5	-	-	Novosphingobium aromaticivorans

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9.5	-	Novosphingobium aromaticivorans

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Novosphingobium aromaticivorans
NADP+	activity 25fold lower than with NAD+	Novosphingobium aromaticivorans

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Release 2023.1 (January 2023)