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Literature summary for 1.3.1.25 extracted from

  • Neidle, E.L.; Shapiro, M.K.; Ornston, L.N.
    Cloning and expression in Escherichia coli of Acinetobacter calcoaceticus genes for benzoate degradation (1987), J. Bacteriol., 169, 5496-5503.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type benD gene and native mutant genes in Escherichia coli and Acinetobacter calcoaceticus Acinetobacter calcoaceticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
2 * 31000, SDS-PAGE Acinetobacter calcoaceticus
62000
-
gel filtration Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Acinetobacter calcoaceticus enzyme in the pathway of dissimilation of benzoate via catechol catechol + CO2 + NADH
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
wild-type ADP1 and spontaneous mutants ADP136, ADP137, ADP138
-

Purification (Commentary)

Purification (Comment) Organism
partial, recombinant from Escherichia coli and Acinetobacter calcoaceticus Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
wild-type enzyme, native and recombinant, and mutants, activity depending on growth substrates Acinetobacter calcoaceticus
108
-
-
Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
-
Acinetobacter calcoaceticus catechol + CO2 + NADH
-
?
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ enzyme in the pathway of dissimilation of benzoate via catechol Acinetobacter calcoaceticus catechol + CO2 + NADH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 31000, SDS-PAGE Acinetobacter calcoaceticus

Synonyms

Synonyms Comment Organism
DHB dehydrogenase
-
Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Acinetobacter calcoaceticus