Cloned (Comment) | Organism |
---|---|
expression of wild-type benD gene and native mutant genes in Escherichia coli and Acinetobacter calcoaceticus | Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000, SDS-PAGE | Acinetobacter calcoaceticus |
62000 | - |
gel filtration | Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | Acinetobacter calcoaceticus | enzyme in the pathway of dissimilation of benzoate via catechol | catechol + CO2 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
wild-type ADP1 and spontaneous mutants ADP136, ADP137, ADP138 | - |
Purification (Comment) | Organism |
---|---|
partial, recombinant from Escherichia coli and Acinetobacter calcoaceticus | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type enzyme, native and recombinant, and mutants, activity depending on growth substrates | Acinetobacter calcoaceticus |
108 | - |
- |
Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | - |
Acinetobacter calcoaceticus | catechol + CO2 + NADH | - |
? | |
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | enzyme in the pathway of dissimilation of benzoate via catechol | Acinetobacter calcoaceticus | catechol + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms | Comment | Organism |
---|---|---|
DHB dehydrogenase | - |
Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acinetobacter calcoaceticus |